prikaz prve stranice dokumenta Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
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Scientific paper - Original scientific paper
Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
Bosnian Journal of Basic Medical Sciences, 22 (2022), 4; 560-568. https://doi.org/10.17305/bjbms.2021.6584
Mužić Radović, Vedrana; Bunoza, Paula; Marić, Tihana; Himelreich-Perić, Marta; Bulić-Jakuš, Floriana; Takahashi, Marta; Jurić-Lekić, Gordana; Sinčić, Nino; Ježek, Davor; Katušić-Bojanac, Ana

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Mužić Radović, V., Bunoza, P., Marić, T., Himelreich Perić, M., Bulić Jakuš, F., Takahashi, M. ... Katušić Bojanac, A. (2022). Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system. Bosnian Journal of Basic Medical Sciences, 22. (4), 560-568. doi: 10.17305/bjbms.2021.6584

Mužić Radović, Vedrana, et al. "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system." Bosnian Journal of Basic Medical Sciences, vol. 22, no. 4, 2022, pp. 560-568. https://doi.org/10.17305/bjbms.2021.6584

Mužić Radović, Vedrana, Paula Bunoza, Tihana Marić, Marta Himelreich Perić, Floriana Bulić Jakuš, Marta Takahashi, Gordana Jurić Lekić, Nino Sinčić, Davor Ježek and Ana Katušić Bojanac. "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system." Bosnian Journal of Basic Medical Sciences 22, no. 4 (2022): 560-568. https://doi.org/10.17305/bjbms.2021.6584

Mužić Radović, V., et al. (2022) 'Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system', Bosnian Journal of Basic Medical Sciences, 22(4), pp. 560-568. doi: 10.17305/bjbms.2021.6584

Mužić Radović V, Bunoza P, Marić T, Himelreich Perić M, Bulić Jakuš F, Takahashi M, and sur.. Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system. Bosnian Journal of Basic Medical Sciences [Internet]. 2022 June 29 [cited 2025 February 17];22(4):560-568. doi: 10.17305/bjbms.2021.6584

V. Mužić Radović, et al., "Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system", Bosnian Journal of Basic Medical Sciences, vol. 22, no. 4, pp. 560-568, June 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:189453. [Accessed: 17 February 2025]

Metadata
Title (english)Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system
AuthorVedrana Mužić Radović
AuthorPaula Bunoza
AuthorTihana Marić
AuthorMarta Himelreich-Perić
AuthorFloriana Bulić-Jakuš
AuthorMarta Takahashi
AuthorGordana Jurić-Lekić
AuthorNino Sinčić
AuthorDavor Ježek
AuthorAna Katušić-Bojanac
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
Although DNA methylation epigenetically regulates development, data on global DNA methylation during development of limb buds (LBs) are scarce. We aimed to investigate the global DNA methylation developmental dynamics in rat LBs cultivated in a serum-supplemented (SS) and in chemically defined serum- and protein-free (SF) three-dimensional organ culture. Fischer rat front- and hind-LBs at 13th and 14th gestation days (GD) were cultivated at the air-liquid interface in Eagle's Minimal Essential Medium (MEM) or MEM with 50% rat serum for 14 days, as SF and SS conditions, respectively. The methylation of repetitive DNA sequences (SINE rat ID elements) was assessed by pyrosequencing. Development was evaluated by light microscopy and extracellular matrix glycosaminoglycans staining by Safranin O. Upon isolation, weak Safranin O staining was present only in more developed GD14 front-LBs. Chondrogenesis proceeded well in all cultures towards day 14, except in the SF-cultivated GD13 hind-LBs, where Safranin O staining was almost absent on day 3. That was associated with a higher percentage of DNA methylation than in SF-cultivated GD13 front-LBs on day three. In SF-cultivated front-LBs, a significant methylation increase between the 3rd and 14th day was detected. In SS-cultivated GD13 front-LBs, methylation increased significantly on day three and then decreased. In older GD14 SS-cultivated LBs, there was no increase of DNA methylation, but they were significantly hypomethylated relative to the SS-cultivated GD13 at days 3 and 14. We confirmed that the global DNA methylation increase is associated with less developed limb organ primordia that strive towards differentiation in vitro, which is of importance for regenerative medicine strategies.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBosnian Journal of Basic Medical Sciences
Numberingvol. 22, no. 4, pp. 560-568
p-ISSN1512-8601
e-ISSN1840-4812
DOIhttps://doi.org/10.17305/bjbms.2021.6584
URN:NBNurn:nbn:hr:105:189453
Publication2022-06-29
Document URLhttps://www.bjbms.org/ojs/index.php/bjbms/article/view/6584
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-10-19 11:10:42