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doctoral thesis
TLR7 gen i protein u karcinomu pluća nemalih stanica
Zagreb: University of Zagreb, School of Medicine, 2022. urn:nbn:hr:105:438442
Džubur, Fedža
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Džubur, F. (2022). TLR7 gen i protein u karcinomu pluća nemalih stanica (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:438442

Džubur, Fedža. "TLR7 gen i protein u karcinomu pluća nemalih stanica." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:438442

Džubur, Fedža. "TLR7 gen i protein u karcinomu pluća nemalih stanica." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:438442

Džubur, F. (2022). 'TLR7 gen i protein u karcinomu pluća nemalih stanica', Doctoral thesis, University of Zagreb, School of Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:438442

Džubur F. TLR7 gen i protein u karcinomu pluća nemalih stanica [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:438442

F. Džubur, "TLR7 gen i protein u karcinomu pluća nemalih stanica", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:105:438442

Metadata
TitleTLR7 gen i protein u karcinomu pluća nemalih stanica
Title (english)TLR7 gene and protein in non-small cell lung cancer
AuthorFedža Džubur
MentorMiroslav Samaržija (mentor)
MentorJelena Knežević (komentor)
Committee memberRajko Kušec (predsjednik povjerenstva)
Committee memberMarko Jakopović (član povjerenstva)
Committee memberMonika Ulamec (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2022-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Cytology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
UVOD: Karcinom pluća vodeći je uzrok smrti od malignih bolesti širom svijeta. Kronična upala, kronična izloženost štetnim tvarima, narušena funkcija urođene i stečene imunosti uz genetske mutacije dovode do promjene u staničnom mikrookolišu i smatraju se najčešćim uzrokom ove teške bolesti. Ovim istraživanjem želimo dokazati povezanost genetičkih biomarkera (polimorfizama) s nastankom i razvojem karcinoma pluća nemalih stanica (NSCLC). ISPITANICI I METODE: U istraživanje je uključeno 450 ispitanika s dijagnozom NSCLC-a te 1283 zdravih ispitanika. Genotipizacijom je određena učestalost polimorfizama TLR7 gena i njhova povezanost s TNM statusom. Metodom RT-PCR ispitana je razina ekspresije TLR7 gena i IFNγ u uzorcima tumorskog tkiva uz analizu limfocita u tumorskom mikrookolišu. REZULTATI: U ispitivanoj populaciji pronašli smo dva najčešća polimofizma TLR7 gena; rs5935436 CT i rs12843803 TG koji su povezani s nastankom NSCLC-a. Prisutnost polimorfnog biljega rs12843803 TG statistički je značajno povezana sa stupnjem zahvaćenosti limfnih čvorova. Rezultati analize profiliranja tumorskog mikrookoliša pokazali su da je stupanj infiltracije CD8+ limfocita T u tumorsku stromu statistički značajno povišen o genotipu rs5935436 CT. ZAKLJUČAK: Rezultati ovog istraživanja pokazuju povezanost nasljedno uvjetovane povećane ekspresije TLR7 gena, rizika za razvoj karcinoma pluća nemalih stanica i obilježja tumorskog okoliša, što može poslužiti kao osnova za daljnja istraživanja protutumorske terapije usmjerene na funkciju TLR7 gena.
Abstract (english)
INTRODUCTION: Lung cancer is the leading cause of death in the group of malignant diseases worldwide. Chronic inflammation, chronic exposure to harmful substances, impaired innate and acquired immune function with genetic mutations lead to changes in the cellular microenvironment and they are considered to be the most common cause of this severe disease. We want to prove the association of cancergenetic biomarkers (polymorphisms) with non-small cell lung cancer (NSCLC). SUBJECTS AND METHODS: The study included 450 patients with NSCLC and 1283 healthy respondents. We used genotyping to determine the frequency of TLR7 gene polymorphisms and their association with TNM status. The level of TLR7 gene and IFNγ expression in tumor tissue samples were examined by RT-PCR with analysis of the tumor microenvironment. RESULTS: In our study rs5935436 CT and rs12843803 TG were the most common polymorphisms of the TLR7 gene associated with the formation of NSCLC. The presence of the polymorphic marker rs12843803 TG was significantly associated with the degree of lymph node metastases. We analysed the tumor microenvironment and showed that the degree of CD8 infiltration into the tumor stroma was significantly higher in patients with rs5935436 CT genotype. CONCLUSION: This study confirmed an association between hereditary increased TLR7 gene expression, risk of non-small cell lung cancer and tumor microenvironment. This data can be useful for the further research on anticancer therapy focused on TLR7 gene function.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:438442
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent81 str.
File originBorn digital
Access conditionsOpen access
Embargo expiration date: 2023-09-19
Terms of useLicence In copyright icon
Created on2022-10-26 09:17:05