prikaz prve stranice dokumenta Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
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Scientific paper - Original scientific paper
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
Scientific Reports, 12 (2022), 1; 1237. https://doi.org/10.1038/s41598-022-05315-7
Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios-Gutiérrez, Juan-José; Tudó, Griselda; Bou, Germán; Ceyssens, Pieter-Jan; Garrigó, Montserrat; González-Martin, Julià; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla-Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B.; Quiroga, Lidia; Ruiz-Serrano, Maria Jesús; Rodríguez-Sánchez, Belén More authors...

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Rodriguez Temporal, D., Alcaide, F., Mareković, I., O’Connor, J. A., Gorton, R., van Ingen, J. ... Rodríguez Sánchez, B. (2022). Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports, 12. (1). doi: 10.1038/s41598-022-05315-7

Rodriguez Temporal, David, et al. "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification." Scientific Reports, vol. 12, no. 1, 2022. https://doi.org/10.1038/s41598-022-05315-7

Rodriguez Temporal, David, Fernando Alcaide, Ivana Mareković, James Anthony O’Connor, Rebecca Gorton, Jakko van Ingen, An Van den Bossche, et al. "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification." Scientific Reports 12, no. 1 (2022). https://doi.org/10.1038/s41598-022-05315-7

Rodriguez Temporal, D., et al. (2022) 'Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification', Scientific Reports, 12(1). doi: 10.1038/s41598-022-05315-7

Rodriguez Temporal D, Alcaide F, Mareković I, O’Connor JA, Gorton R, van Ingen J, and sur.. Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports [Internet]. 2022 January 24 [cited 2024 May 04];12(1). doi: 10.1038/s41598-022-05315-7

D. Rodriguez Temporal, et al., "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification", Scientific Reports, vol. 12, no. 1, January 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:313006. [Accessed: 04 May 2024]

Metadata
Title (english)Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
AuthorDavid Rodriguez-Temporal
AuthorFernando Alcaide
AuthorIvana Mareković
AuthorJames Anthony O’Connor
AuthorRebecca Gorton
AuthorJakko van Ingen
AuthorAn Van den Bossche
AuthorGenevieve Héry-Arnaud
AuthorClémence Beauruelle
AuthorDorothea Orth-Höller
AuthorJuan-José Palacios-Gutiérrez
AuthorGriselda Tudó
AuthorGermán Bou
AuthorPieter-Jan Ceyssens
AuthorMontserrat Garrigó
AuthorJulià González-Martin
AuthorGilbert Greub
AuthorJaroslav Hrabak
AuthorAndré Ingebretsen
AuthorMaria Concepción Mediavilla-Gradolph
AuthorMarina Oviaño
AuthorBegoña Palop
AuthorArthur B. Pranada
AuthorLidia Quiroga
AuthorMaria Jesús Ruiz-Serrano
AuthorBelén Rodríguez-Sánchez
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleScientific Reports
Numberingvol. 12, no. 1, 1237
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-022-05315-7
URN:NBNurn:nbn:hr:105:313006
Publication2022-01-24
Document URLhttps://www.nature.com/articles/s41598-022-05315-7
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-11-14 12:35:18