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doctoral thesis
Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate
Zagreb: University of Zagreb, School of Medicine, 2022. urn:nbn:hr:105:267062
Škara, Lucija
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Škara, L. (2022). Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:267062

Škara, Lucija. "Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:267062

Škara, Lucija. "Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:267062

Škara, L. (2022). 'Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate', Doctoral thesis, University of Zagreb, School of Medicine, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:267062

Škara L. Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2022 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:267062

L. Škara, "Metilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:105:267062

Metadata
TitleMetilacija nestaničnoga DNA gena RASSF1 i CAV1 u krvi i ejakulatu bolesnika s rakom prostate
Title (english)Cell-free DNA methylation of RASSF1 and CAV1 genes in blood and ejaculate of patients with prostate cancer
AuthorLucija Škara
MentorMonika Ulamec (mentor)
Committee memberAna Katušić Bojanac (predsjednik povjerenstva)
Committee memberIvan Šamija (član povjerenstva)
Committee memberBorislav Spajić (član povjerenstva)
Committee memberMilan Milošević (član povjerenstva)
Committee memberTomislav Kelava (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2022-10-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Urology
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Metode ranog otkrivanja raka prostate su digitorektalni pregled i povišene serumske vrijednosti antigena specifičnog za prostatu (PSA). Obzirom da PSA nije tumor specifičan marker te je povišen i u drugim stanjima (npr. benignoj hiperplaziji prostate (BHP) za definitivnu dijagnozu potrebna je biopsija koja kod samo trećine pacijenata potvrdi dijagnozu. Zbog svega toga potrebni su novi biomarkeri za otkrivanje raka prostate, posebno oni koji se mogu određivati u tekućinskim biopsijama. Kako su geni RASSF1A i CAV1 u tkivu raka prostate hipermetilirani u odnosu na tkivo BHP-a, istražio se potencijal metilacije gena RASSF1 i CAV1 u cfDNA iz uzoraka krvi i ejakulata kao markera za razlikovanje raka prostate od BHP-a. Ekspresija odabranih gena na proteinskoj razini bila je različita među skupinama. Metilacija gena RASSF1A pokazala se dobrim markerom za razlikovanje tkiva raka prostate od tkiva BHP-a, ali ne i za razlikovanje pacijenata s rakom prostate i BHP-om na temelju analize cfDNA u tekućinskim biopsijama. Metilacija gena CAV1 pokazala se dobrim markerom za razlikovanje pacijenata s rakom prostate od pacijenata s BHP-om, budući da je metilacija pozicije CpG1 gena CAV1 bila značajno niža u ejakulatu pacijenata s rakom prostate od pacijenata s BHP-om. Metilacija gena CAV1 na ostalim CpG mjestima kao i metilacija u krvi se nije razlikovala između skupina. Temeljem rezultata ove disertacije može se zaključiti da je metilacija gena CAV1 na mjestu CpG1 ejakulata potencijalni biomarker za rak prostate.
Abstract (english)
Early prostate cancer detection methods are digital rectal examination and elevated prostate-specific antigen (PSA) levels. As PSA is not cancer specific and increased level may be caused by other conditions (e.g. benign prostatic hyperplasia (BPH)), for definitive diagnosis biopsy is needed, which confirms the diagnosis for only one third of patients. Because of all this, new biomarkers for prostate cancer detection are needed, especially those available in liquid biopsy samples. Since RASSF1 and CAV1 genes are hypermethylated in prostate cancer tissue compared to BPH tissue, potential of RASSF1 and CAV1 gene methylation of cfDNA from blood and ejaculate samples as a biomarker to distinguish between PC and BPH was assessed. Expression of selected genes on protein level differed between groups. RASSF1A gene methylation distinguished well prostate cancer tissue from BPH tissue but cfDNA methylation did not differ between groups. CAV1 gene methylation distinguished well prostate cancer patients from BPH patients since CpG1 methylation of CAV1 gene in ejaculate cfDNA was significantly lower in prostate cancer group. CAV1 gene methylation on other CpG sites in ejaculate or blood cfDNA did not differ between groups. To conclude, methylation CpG1site of CAV1 gene in ejaculate has the potential as a biomarker for prostate cancer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:267062
ProjectNumber: KK.01.1.1.01.0008
Title: Reproduktivna i regenerativna medicina - istraživanje novih platformi i potencijala
Leader: Ana Katušić Bojanac
Jurisdiction: eu
Funder: MZO
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent90 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-29 11:55:41