prikaz prve stranice dokumenta A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A
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Scientific paper - Original scientific paper
A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A
Pathogens, 11 (2022), 4; 482. https://doi.org/10.3390/pathogens11040482
Mitteregger, Dieter; Wessely, Julian; Barišić, Ivan; Bedenić, Branka; Kosak, Dieter; Kundi, Michael

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Mitteregger, D., Wessely, J., Barišić, I., Bedenić, B., Kosak, D. & Kundi, M. (2022). A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A. Pathogens, 11. (4). doi: 10.3390/pathogens11040482

Mitteregger, Dieter, et al. "A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A." Pathogens, vol. 11, no. 4, 2022. https://doi.org/10.3390/pathogens11040482

Mitteregger, Dieter, Julian Wessely, Ivan Barišić, Branka Bedenić, Dieter Kosak and Michael Kundi. "A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A." Pathogens 11, no. 4 (2022). https://doi.org/10.3390/pathogens11040482

Mitteregger, D., et al. (2022) 'A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A', Pathogens, 11(4). doi: 10.3390/pathogens11040482

Mitteregger D, Wessely J, Barišić I, Bedenić B, Kosak D, Kundi M. A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A. Pathogens [Internet]. 2022 April 18 [cited 2024 April 28];11(4). doi: 10.3390/pathogens11040482

D. Mitteregger, J. Wessely, I. Barišić, B. Bedenić, D. Kosak and M. Kundi, "A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A", Pathogens, vol. 11, no. 4, April 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:048963. [Accessed: 28 April 2024]

Metadata
Title (english)A Variant Carbapenem Inactivation Method (CIM) for Acinetobacter baumannii Group with Shortened Time-to-Result: rCIM-A
AuthorDieter Mitteregger
AuthorJulian Wessely
AuthorIvan Barišić
AuthorBranka Bedenić
AuthorDieter Kosak
AuthorMichael Kundi
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Microbiology and Parasitology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract (english)
Carbapenem-resistant Acinetobacter baumannii group organisms (CRAB) are challenging because the choice between targeted, new antibiotic drug options and hygiene measures should be guided by a timely identification of resistance mechanisms. In CRAB, acquired class-D carbapenemases (CHDLs) are active against meropenem and imipenem. If PCR methods are not the first choice, phenotypic methods have to be implemented. While promising, the carbapenemase inactivation method (CIM) using meropenem-hydrolysis is, however, hampered by poor performance or overly long time-to-result. We developed a rapid CIM (rCIM-A) with good performance using ertapenem, imipenem, and meropenem disks, 2-h permeabilization and incubation with the test strain in trypticase soy broth, and a read-out of residual carbapenem activity after 6 h, and optionally after 16–18 h. Using clinical isolates and type-strains of Acinetobacter (n = 67) not harboring carbapenemases (n = 28) or harboring acquired carbapenemases (n = 39), the sensitivity of detection was 97.4% with the imipenem disk after 6 h at a specificity of 92.9%. If the inhibition zone around the ertapenem disk at 6 h was 6 or ≤26 mm at 16–18 h, or ≤25.5 mm for meropenem, the specificity was 100%. Because of the high negative predictive value, the rCIM-A seems particularly appropriate in areas of lower CRAB-frequency.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titlePathogens
Numberingvol. 11, no. 4, 482
e-ISSN2076-0817
DOIhttps://doi.org/10.3390/pathogens11040482
URN:NBNurn:nbn:hr:105:048963
Publication2022-04-18
Document URLhttps://www.mdpi.com/2076-0817/11/4/482
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2022-12-12 08:10:47