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doctoral thesis
Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva
Zagreb: University of Zagreb, School of Medicine, 2022. urn:nbn:hr:105:203537
Barišić, Ana
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Barišić, A. (2022). Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:203537

Barišić, Ana. "Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:203537

Barišić, Ana. "Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:203537

Barišić, A. (2022). 'Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva', Doctoral thesis, University of Zagreb, School of Medicine, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:203537

Barišić A. Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2022 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:105:203537

A. Barišić, "Presječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:105:203537

Metadata
TitlePresječno istraživanje koncentracije grelina, leptina i proupalnih citokina u serumu bolesnika s upalnim bolestima crijeva
Title (english)Cross sectional study of serum ghrelin, leptin and proinflammatory cytokines levels in inflammatory bowel disease
AuthorAna Barišić
MentorŽeljko Krznarić (mentor)
Committee memberNeven Ljubičić (predsjednik povjerenstva)
Committee memberMarko Banić (član povjerenstva)
Committee memberŽarko Babić (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2022-11-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Upalne bolesti crijeva (UBC) kronične su bolesti čiji se porast incidencija prati u posljednjih nekoliko godina. Stoga ne čudi da je velik broj istraživanja u UBC-u usmjeren ka otkrivanju biomarkera koji bi bio koristan u praćenju aktivnosti bolesti. Kako u upalnim bolestima crijeva dolazi do čitavog niza metaboličkih promjena, moguće su fluktuacije proizvodnje grelina i leptina, te bi upravo njihove serumske razine mogle predstavljati potencijalne biomarkere u UBC-u. Osim toga, citokini su često istraživani biomarkeri u UBC-u, s obzirom da su upravo oni ključne komponente u upalnim putevima važnima u patogenezi UBC-a. Cilj našeg istraživanja bio je odrediti serumske koncentracije grelina, leptina, TNF-α i IL-6 u UBC-u te istražiti povezanost njihovih razina s aktivnosti bolesti i nutritivnim statusom. Rezultati istraživanja su pokazali da bolesnici s UBC-om imaju niže vrijednosti leptina, a više vrijednosti TNF-α u odnosu na kontrolnu skupinu. Dokazana je pozitivna korelacija IL-6 s laboratorijskim pokazateljima upalne aktivnosti, te s kliničkom aktivnosti UBC-a. Osim toga, dokazali smo pozitivnu korelaciju leptina s masom masnog tkiva, stopom visceralnog masnog tkiva te indeksom tjelesne mase te njegovu negativnu korelaciju sa skeletnom mišićnom masom u UBC-u. Razine grelina u većini naših uzoraka bile su nedetektabilne, što smo objasnili greškom u metodologiji. U našem istraživanju IL-6 se pokazao kao potencijalni biomarker u praćenju aktivnosti bolesti, a leptin kao dobar pokazatelj nutritivnog statusa. Međutim, potencijalnu ulogu ispitivanih biomarkera u upalnim bolestima crijeva potrebno je reevaluirati na većem i homogenijem uzorku ispitanika.
Abstract (english)
Inflammatory bowel disease (IBD) is a chronic disease with an increasing incidence in the past few years. Much research in the IBD field has focused on finding reliable serum biomarkers for assessing disease activity. IBD is associated with several metabolic changes that may lead to fluctuation of ghrelin and leptin production. Therefore, serum ghrelin and leptin levels may represent potential biomarkers in IBD. Furthermore, cytokines are commonly researched biomarkers in IBD, due to the fact they have been identified as the key players in intestinal inflammation. Our research has aimed to investigate serum ghrelin, leptin, TNF-α, and IL-6 concentrations in IBD and the association between their levels and activity of the disease and nutritional status. Our results suggest that patients with IBD have lower levels of leptin, and higher levels of TNF-α in comparison with the control group. It has been shown that IL-6 positively correlates with laboratory and clinical markers of disease activity. In addition, leptin levels have been found to exhibit a positive correlate with levels of fat mass, visceral fat mass, and body mass index. A negative correlation between leptin levels and skeletal muscle mass in IBD patients has been proven. Systematic measurement errors are a probable cause of undetectable levels of ghrelin in our research. Finally, IL-6 has been shown as a potential biomarker of disease activity. However, additional studies with larger homogenous cohorts may be needed to validate the value of tested biomarkers as non-invasive biomarkers of IBD activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:203537
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent99 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-01-12 09:57:44