prikaz prve stranice dokumenta Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity
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doctoral thesis
Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity
Zagreb: University of Zagreb, School of Medicine, 2022. urn:nbn:hr:105:643274
Sreter, Katherina Bernadette
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Sreter, K. B. (2022). Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:643274

Sreter, Katherina Bernadette. "Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:643274

Sreter, Katherina Bernadette. "Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity." Doctoral thesis, University of Zagreb, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:105:643274

Sreter, K. B. (2022). 'Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity', Doctoral thesis, University of Zagreb, School of Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:643274

Sreter KB. Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:643274

K. B. Sreter, "Association of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:105:643274

Metadata
TitleAssociation of platelet serotonin, plasma brain-derived neurotrophic factor (BDNF) and Val66Met BDNF gene polymorphism with asthma severity
Title (croatian)Povezanost trombocitnoga serotonina, moždanoga neurotrofnoga čimbenika (BDNF) u plazmi te Val66Met polimorfizma gena BDNF sa stupnjem težine astme
AuthorKatherina Bernadette Sreter
MentorSanja Popović-Grle (mentor)
MentorDubravka Švob Štrac (komentor)
Committee memberJasenka Markeljević (predsjednik povjerenstva)
Committee memberMiroslav Samaržija (član povjerenstva)
Committee memberNela Pivac (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2022-12-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Universal decimal classification
(UDC)		615 - Pharmacology. Therapeutics. Toxicology
Abstract
Asthma is a common but complex chronic inflammatory disease of the respiratory airways. Serotonin (5-HT) and brain-derived neurotrophic factor (BDNF) are associated with regulation of lung function and immune responses; however, their exact involvement in the pathophysiology of asthma remains unclear. We investigated platelet 5-HT and plasma BDNF concentrations, platelet monoamine oxidase B (MAO-B) activity, and the distribution of genetic variants of BDNF rs6265 (Val66Met) and neurotrophic receptor tyrosine kinase 2 (NTRK2) rs1439050 polymorphisms in 120 asthma patients and 120 healthy subjects, and evaluated their relationship with asthma severity and phenotype. Platelet 5-HT concentrations were lower, whereas platelet MAO-B activity and plasma BDNF concentrations were higher in asthma patients. No differences were observed in these parameters between subjects with asthma subdivided according to asthma severity or phenotype. However, asthma patients with aspirin sensitivity and aspirin-exacerbated respiratory disease had higher plasma BDNF levels. The distribution of BDNF Val66Met and NTRK2 rs1439050 genetic variants showed no differences between subjects with asthma and healthy individuals, or between patients with different asthma severity and phenotype. The BDNF Val66Met polymorphism, but not NTRK2 rs1439050 polymorphism, influenced plasma BDNF levels in healthy subjects only. These results suggest that 5-HT and MAO-B in platelets as well as BDNF in plasma may serve as potential peripheral biomarkers for asthma but not asthma severity.
Abstract (croatian)
Astma je česta ali kompleksna kronična upalna bolest dišnih putova. Serotonin (5-HT) i moždani neurotrofni čimbenik (BDNF) su povezani s regulacijom funkcije pluća i imunoloških odgovora; međutim, njihova točna uloga u patofiziologiji astme ostaje nerazjašnjena. Istražili smo koncentracije trombocitnog 5-HT-a i BDNF-a u plazmi, aktivnost trombocitne monoaminooksidaze B (MAO-B), i raspodjelu genskih varijanti BDNF rs6265 (Val66Met) polimorfizma i NTRK2 (gen za neurotrofni receptor tirozin kinaza 2) rs1439050 polimorfizma u 120 pacijenata s astmom i 120 zdravih osoba, te istražili njihovu povezanost sa stupnjem težine i fenotipom astme. Koncentracije trombocitnog 5-HT-a bile su snižene, dok su aktivnost trombocitne MAO-B i koncentracije BDNF-a u plazmi bile povišene u pacijenata s astmom. Nisu opažene razlike u ovim parametrima između osoba oboljelih od astme podijeljenih prema težini bolesti ili fenotipu. Međutim, pacijenti s aspirin-osjetljivom astmom i aspirin-pogoršanom respiratornom bolešću imali su povišene razine BDNF-a u plazmi. Raspodjela genskih varijanti BDNF Val66Met i NTRK2 rs1439050 polimorfizama nije pokazala razliku niti između pacijenata s astmom i zdravih osoba niti između oboljelih različitih stupnjeva težine i fenotipova astme. BDNF Val66Met polimorfizam, ali ne i NTRK2 rs1439050 polimorfizam, utjecao je na razine BDNF-a u plazmi samo zdravih ispitanika. Ovi rezultati ukazuju da 5-HT i MAO-B u trombocitima, te BDNF u plazmi mogu poslužiti kao potencijalni periferni biomarkeri za astmu, ali ne i za težinu astme.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:105:643274
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent163 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-01-30 12:13:54