Tear film dysfunction or dry eye disease (DED) is a multifactorial and frequent disease that results in symptoms of discomfort and visual disturbances resulting in possible damage to the surface of the eye. Currently not fully standardized test for diagnosis and monitoring of disease flow. The tests are in most cases inaccurate and have low repeatability. Current methods of diagnosing include examination on slit lamp, determination of the tear breakup time, evaluation of the function of the Meibomian glands, estimation of the tear meniscus as well as the most common Schirmer test. Without a diagnostic standard to monitor the disease, treatment of dry eye diseases is usually symptomatic, i.e. essentially on a well-taken medical history. Because of all of the above, the disease of the dry eye becomes a growing public health problem because it can significantly affect the vision of a person and lead to a decrease in quality of life. Through this graduate thesis we conducted a study in which we used optical coherence tomography of tear meniscus as a method of detection of dry eye disease. The survey included 18 respondents, who fulfilled the OSDI questionnaire, accessed ophthalmological examination and tear meniscus measurements with optical coherence tomography. The study demonstrated the statistical correlation of the examined variables- tear meniscus surface area (P = 0.036), the native tear break- up time (p = 0.030) and the tear break- up time after the expression of the Meibomian glands (p = 0.028).