prikaz prve stranice dokumenta Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium
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Scientific paper - Original scientific paper
Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium
Anatomia, Histologia, Embryologia, 51 (2022), 5; 592-601. https://doi.org/10.1111/ahe.12835
Himelreich Perić, Marta; Mužić‐Radović, Vedrana; Marić, Tihana; Bulić‐Jakuš, Floriana; Jurić‐Lekić, Gordana; Takahashi, Marta; Sinčić, Nino; Ježek, Davor; Katušić‐Bojanac, Ana

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Himelreich Perić, M., Mužić‐Radović, V., Marić, T., Bulić‐Jakuš, F., Jurić‐Lekić, G., Takahashi, M. ... Katušić‐Bojanac, A. (2022). Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium. Anatomia, Histologia, Embryologia, 51. (5), 592-601. doi: 10.1111/ahe.12835

Himelreich Perić, Marta, et al. "Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium." Anatomia, Histologia, Embryologia, vol. 51, no. 5, 2022, pp. 592-601. https://doi.org/10.1111/ahe.12835

Himelreich Perić, Marta, Vedrana Mužić‐Radović, Tihana Marić, Floriana Bulić‐Jakuš, Gordana Jurić‐Lekić, Marta Takahashi, Nino Sinčić, Davor Ježek and Ana Katušić‐Bojanac. "Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium." Anatomia, Histologia, Embryologia 51, no. 5 (2022): 592-601. https://doi.org/10.1111/ahe.12835

Himelreich Perić, M., et al. (2022) 'Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium', Anatomia, Histologia, Embryologia, 51(5), pp. 592-601. doi: 10.1111/ahe.12835

Himelreich Perić M, Mužić‐Radović V, Marić T, Bulić‐Jakuš F, Jurić‐Lekić G, Takahashi M, and sur.. Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium. Anatomia, Histologia, Embryologia [Internet]. 2022 July 11 [cited 2024 November 23];51(5):592-601. doi: 10.1111/ahe.12835

M. Himelreich Perić, et al., "Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium", Anatomia, Histologia, Embryologia, vol. 51, no. 5, pp. 592-601, July 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:145714. [Accessed: 23 November 2024]

Metadata
Title (english)Transmembranous and enchondral osteogenesis in transplants of rat limb buds cultivated in serum‐ and protein‐free culture medium
AuthorMarta Himelreich Perić
AuthorVedrana Mužić‐Radović
AuthorTihana Marić
AuthorFloriana Bulić‐Jakuš
AuthorGordana Jurić‐Lekić
AuthorMarta Takahashi
AuthorNino Sinčić
AuthorDavor Ježek
AuthorAna Katušić‐Bojanac
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract (english)
Cartilage differentiates in rat limb buds cultivated in a chemically defined protein-free medium in the same manner as in the richer serum-supplemented medium. We aimed to investigate the remaining differentiation potential of pre-cultivated limb buds by subsequent transplantation in vivo. Rat front (FLBs) and hind-limb buds (HLBs) were isolated from Fischer rat dams at the 14th gestation day (GD 14) and cultivated at the air-liquid interface in Eagle's Minimum Essential Medium (MEM) alone; with 5 μM of 5-azacytidine (5azaC) or with rat serum (1:1). Overall growth was measured seven times during the culture by an ocular micrometre. After 14 days, explants were transplanted under the kidney capsule of adult males. Growth of limb buds was significantly lower in all limb buds cultivated in MEM than in those cultivated with serum. In MEM with 5azaC, growth of LBs was significantly lower only on day 3 of culture. Afterwards, it was higher throughout the culture period, although a statistically significant difference was assessed only for HLBs. In transplants, mixed structures developed with the differentiated transmembranous bone, cartilage with enchondral ossification, bone-marrow, sebaceous gland, and hair that have never been found in vitro. Nerves differentiated only in transplants precultivated in the serum-supplemented medium. We conclude that pre-cultivation of LBs in a chemically defined protein-free medium does not restrict osteogenesis and formation of epidermal appendages but is restrictive for neural tissue. These results are important for understanding limb development and regenerative medicine strategies.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleAnatomia, Histologia, Embryologia
Numberingvol. 51, no. 5, pp. 592-601
p-ISSN0340-2096
e-ISSN1439-0264
DOIhttps://doi.org/10.1111/ahe.12835
URN:NBNurn:nbn:hr:105:145714
Publication2022-07-11
Document URLhttps://onlinelibrary.wiley.com/doi/10.1111/ahe.12835
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-03-28 11:56:45