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doctoral thesis
Utjecaj serotoninemije na metabolizam koštane pregradnje
Zagreb: University of Zagreb, School of Medicine, 2014. urn:nbn:hr:105:713744
Erjavec, Igor
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Erjavec, I. (2014). Utjecaj serotoninemije na metabolizam koštane pregradnje (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:713744

Erjavec, Igor. "Utjecaj serotoninemije na metabolizam koštane pregradnje ." Doctoral thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:713744

Erjavec, Igor. "Utjecaj serotoninemije na metabolizam koštane pregradnje ." Doctoral thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:713744

Erjavec, I. (2014). 'Utjecaj serotoninemije na metabolizam koštane pregradnje ', Doctoral thesis, University of Zagreb, School of Medicine, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:105:713744

Erjavec I. Utjecaj serotoninemije na metabolizam koštane pregradnje [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2014 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:105:713744

I. Erjavec, "Utjecaj serotoninemije na metabolizam koštane pregradnje ", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:105:713744

Metadata
TitleUtjecaj serotoninemije na metabolizam koštane pregradnje
Title (english)Effect of serotoninemia on bone remodeling metabolism
AuthorIgor Erjavec
Mentor Lovorka Grgurević (mentor)
Committee membernije dostupno (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2014-10-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Mehanizam djelovanja serotonina (5-HT) na koštano tkivo nije u potpunosti razjašnjen. U ovom radu istražen je utjecaj promijenjene homeostaze 5-HT-a na koštano tkivo u WZ-5HT štakorskom modelu. Mineralna gustoća i koštani parametri bedrene kosti i 3. lumbalnog kralješka izmjereni su DXA i mikro CT uređajima. Povećana aktivnost 5-HT sustava u WZ-5HT štakora uzrokovala je povećanu tjelesnu masu i rast kostiju. Kod kortikalne kosti primjećeno je povećano periostalno širenje i endostealna resorpcija u životinja iz 5HT-visoke sublinije. Značajno povećana stopa koštanog stvaranja, uz povišenu koncentraciju markera koštane razgradnje, upućuju na povećanje koštane pregradnje u štakora iz 5HT-visoke sublinije. Visoka stopa koštane pregradnje uzrokovala je smanjenje trabekularne kosti distalne bedrene kosti i lumbalnog dijela kralješnice u tih životinja. U štakora iz 5HT-visoke sublinije je cijeljenje prijeloma bilo usporeno zbog smanjene mineralizacije koštanog kalusa. Životinje s povišenom količinom 5-HT-a imale su smanjenu količinu 1,25(OH)2D3 te su starenjem razvile hiperglikemiju, hiperinzulinemiju i povećano nakupljanje visceralnog masnog tkiva, što su indikatori razvoja fenotipa dijabetesa melitusa tipa II. Pojedinačno, 5-HT i inzulin nisu pokazali učinak na osteoblaste i osteoklaste, ali zajedno su sinergistički pojačali diferencijaciju osteoblasta i smanjili diferencijaciju osteoklasta. Pokazano je da 5-HT djeluje na koštano tkivo u sinergiji s inzulinom potičući aktivaciju osteoblasta koji dalje aktiviraju osteoklaste i tako povećavaju koštanu pregradnju.
Abstract (english)
The mechanism of serotonin (5-HT) effect on bone is still unclear. In this research the effect of altered 5-HT homeostasis on bone was further investigated in WZ-5HT rat model. Mineral density and bone parameters of femur and 3rd lumbar vertebrae were resolved using DXA and micro CT, respectively. Elevated blood 5-HT caused increased body weight and bone growth in WZ-5HT rats. Cortical bone had increased periosteal formation and endosteal resorption in animals from 5HT-high subline. Sigificant increase in bone formation rate follwed by increase in bone resorption marker indicate high bone remodelling in high-5HT rats, which decreased trabecular bone at the site of distal femur and lubar spine. Fracture healing was delayed in high-5HT animals due to prolonged mineralization of bone callus. Animals with elevated 5-HT level had decreased level of 1,25(OH)2D3 and they developed hyperglycemia, hyperinsulinemia and increased accumulation of visceral fat tissue with ageing, a symptoms of diabetes melitus type II phenotype development. In osteoblasts and osteoclasts neither 5-HT nor insulin had effect, while their sinergistic effect caused increased differentiation of osteoblasts and decreased differentiation of osteoclasts, respectively. It has been shown that 5-HT exerts its effect on bone together with insulin promoting osteoblast activation which in turn activates osteoclasts and increases bone turnover.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:713744
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
Type of resourceText
Extent102 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-05 07:25:12