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doctoral thesis
Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora
Zagreb: University of Zagreb, School of Medicine, 2016. urn:nbn:hr:105:147542
Zlatar, Mirna
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Zlatar, M. (2016). Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:147542

Zlatar, Mirna. "Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora ." Doctoral thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:147542

Zlatar, Mirna. "Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora ." Doctoral thesis, University of Zagreb, School of Medicine, 2016. https://urn.nsk.hr/urn:nbn:hr:105:147542

Zlatar, M. (2016). 'Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora ', Doctoral thesis, University of Zagreb, School of Medicine, accessed 02 April 2025, https://urn.nsk.hr/urn:nbn:hr:105:147542

Zlatar M. Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2016 [cited 2025 April 02] Available at: https://urn.nsk.hr/urn:nbn:hr:105:147542

M. Zlatar, "Učinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora ", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:105:147542

Metadata
TitleUčinak retrobulbarno apliciranoga pentadekapeptida BPC 157 na djelovanje retrobulbarno aplicirane L-NAME u štakora
Title (english)Effect of retrobulbar application pentadecapeptide BPC 157 on effect of retrobulbar application L-NAME in rats
AuthorMirna Zlatar
MentorPredrag Sikirić (mentor)
Committee membernije dostupno (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2016-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Ženke Wistar štakora, 200g, randomizirano podijeljene, retrobulbarno su tretirane sa: L-NAME (0,1ml/0,2mg/oko) i BPC 157 (0,1ml/0,2μg/oko, 20 minuta nakon primjene L-NAME); L-NAME (0,1ml/0,2mg/oko) i BPC 157 (0,1ml/0,2ng/oko, 20 minuta nakon primjene L-NAME); L-NAME (0,1ml/0,2mg/oko) i 0,9% NaCl (0,1ml/oko, 20 minuta nakon primjene L-NAME). Kontrolne su životinje istovremeno retrobulbarno primale jednaki volumen 0,9% NaCl. USB mikroskopskom kamerom „Veho discovery VMS-004 deluxe“ i „VOLK“... More digital wide field lupom za indirektnu oftalmolskopiju snimana je očna pozadina. Inicijalno, životinje su snimane: prije retrobulbarne aplikacije L-NAME (odnosno 0,9% NaCl); 20 minuta nakon aplikacije L-NAME (odnosno 0,9% NaCl); ponovno nakon aplikacije BPC 157 (odnosno 0,9% NaCl) te nakon 20 minuta. Nadalje snimane su 1. i 2. dan nakon aplikacije te nakon 1., 2. i 4. tjedna (neposredno prije žrtvovanja). Očna je pozadina analizirana računalnim programom za analizu fotografija te patohistološkom analizom bulbusa na standardnim rezovima, uklopljenog u parafin i bojenog hemalaun eozinom. Snimano ponašanje životinja uključivalo je vrijeme imobilnosti životinja nakon stavljanja na nepoznatu podlogu i reakciju životinja na podražaj plavo-zelenom rukavicom. Retrobulbamo aplicirana L-NAME uzrokovala je vazokonstrikciju arterija mrežnice, ishemiju mrežnice te značajnu imobilnost životinja. Retrobulbarna primjena BPC 157 dovela je do oporavka mrežnične ishemije te je spriječila njenu atrofiju. Ponašanje životinja bilo je normalno. Less
Abstract (english)
Female Wistar rats, 200 g, randomly assigned, were treated retrobulbary with: L-NAME (0.1ml/0.2mg/eye) and BPC 157 (0.1ml/0.2μg/eye 20 minutes after L-NAME application); L-NAME (0.1ml/0.2mg/eye) and BPC 157 (0.1ml/0.2ng/eye 20 minutes after L-NAME); L-NAME (0.1ml/0.2 mg/eye) and 0.9% NaCl (0.1ml/eye 20 minutes after L-NAME application). Control animals simultaneously received retrobulbar equivolume of 0.9% NaCl. With a USB microscope camera „Veho discovery VMS-004 deluxe“ and "VOLK"... More digital wide field loupe for indirect ophthalmoscope eye fundus was recorded in the following time periods: before retrobulbar application of L-NAME (or 0.9% NaCl); 20 minutes after L-NAME (or 0.9% NaCl); again after BPC 157 (or 0.9% NaCl), and 20 minutes after. Furthermore, recording was performed at the post-application day 1, 2, and 7, 14, 28 (last day, just before the sacrifice). The fundus was analysed by photo analysis software and by histopathologic eyeball analysis on standard sections, paraffin embedded and hemalaun-eosin stained. The animals behavior was filmed, observing the following parameters: time of immobility and reaction time to visual stimuli (blue-green glove). The retrobulbar application of L-NAME causes the vasoconstriction of the retina's arteries, retinal ischemia, while retrobulbar application of BPC 157 leads to retinal ischemia recovery, prevents its atrophy and restores normal animal behavior. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:147542
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
Type of resourceText
Extent97 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-05 07:52:52
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