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doctoral thesis
Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva
Zagreb: University of Zagreb, School of Medicine, 2018. urn:nbn:hr:105:044531
Panek, Marina
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Panek, M. (2018). Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:044531

Panek, Marina. "Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva ." Doctoral thesis, University of Zagreb, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:105:044531

Panek, Marina. "Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva ." Doctoral thesis, University of Zagreb, School of Medicine, 2018. https://urn.nsk.hr/urn:nbn:hr:105:044531

Panek, M. (2018). 'Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva ', Doctoral thesis, University of Zagreb, School of Medicine, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:105:044531

Panek M. Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2018 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:105:044531

M. Panek, "Sastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva ", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:105:044531

Metadata
TitleSastav crijevne mikrobiote i čimbenici upale u bolesnika s upalnim bolestima crijeva
Title (english)Gut microbiota composition and inflammatory markers in IBD patients
AuthorMarina Panek
MentorDonatella Verbanac (mentor)
MentorŽeljko Krznarić (komentor)
Committee membernije dostupno (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2018-10-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Upalna bolest crijeva predstavlja kronični proces sa sve većom učestalošću koja se dijeli na Crohn-ovu bolest (CD) i ulcerozni kolitis (UC). Nastanak bolesti povezan je sa sastavom crijevne mikrobiote, imunim statusom i unosom hrane. U ovoj studiji analizirana je crijevna mikrobiota u uzorcima stolice i biopsiji sluznice kolona novodijagnosticiranih pacijenata i pripadajućih kontrola metodom sekvenciranja nove generacije, te je utvrđen upalni status svakog pacijenta analizom upalnih proteina u krvi imunodetekcijskom metodom pomoću specifičnih protutijela. Rezultati pokazuju da su promjene u sastavu mikrobiote u fecesu i sluznici kolona specifične i značajne za Crohnovu bolest (koljeno Proteobacteria, Actinobacteria i roda Dialister) i ulcerozni kolitis (porodice Turibacteraceae, Veillonellaceae, Oxalobacteraceae i Sphingomonadaceae). Analizom crijevne mikrobiote utvrđeni su i taksoni na temelju kojih možemo razlikovati CD i UC, a to su koljeno Verrucomicrobia, razred Bacilli i rod Clostridium. Te promjene u sastavu mikrobiote također utječu i na aktivaciju određenih upalnih proteina koji igraju veliku ulogu u kroničnom imunološkom odgovoru. Najznačajnija je razlika u serumskim proteinima CXCL10, IL-6, HGF i IL-17A između CD i IBS pacijenata te razlika u serumskim proteinima CDCP1, FI3L i IL-6 između CD i UC pacijenata. Rezultati provedenih istraživanja u novodijagnosticiranih pacijenata mogu pomoći boljoj stratifikaciji pacijenata i boljem odabiru terapijskih pristupa kod upalnih bolesti crijeva.
Abstract (english)
Inflammatory bowel disease (IBD) is a chronic process in the gastrointestinal tract with increasing prevalence that consist of Crohn disease (CD) and ulcerative colitis (UC). The disease is associated with intestinal microbiota composition, immune status and food intake. In this study microbiota composition in intestinal biopsy samples and stool of newly diagnosed IBD patients and non-IBD individuals, as well as inflammatory status from the blood of each participant was investigated. Microbiota composition was determined by the next generation sequencing method while serum biomarkers were detected by specifically tagged antibodies. Results show that the changes in feces and colon microbiota are specific and significant for Crohn's disease (Proteobacteria, Actinobacteria phylum and Dialister genus) and ulcerative colitis (Turibacteraceae, Veillonellaceae, Oxalobacteraceae and Sphingomonadaceae families). These changes in microbiota composition induce activation of certain inflammatory proteins that play a major role in chronic inflammatory response. The most significant differences in a protein level are in CXCL10, IL-6, HGF and IL-17A between CD and control patients and differences observed in CDCP1, FI3L and IL-6 proteins between CD and UC patients. Results of this translational research on newly diagnosed patients could help in better stratification of patients and selection of more suitable therapeutic approaches in the treatment of inflammatory bowel disease.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:044531
Study programmeTitle: Biomedicine and Health Sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva (doktor/doktorica znanosti, područje biomedicine i zdravstva)
Type of resourceText
Extent83 str.
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-05 08:14:43