prikaz prve stranice dokumenta 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
  Download
PDF 5.99 MB
Scientific paper - Original scientific paper
4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
International Journal of Molecular Sciences, 23 (2022), 22; 14373. https://doi.org/10.3390/ijms232214373
Cindrić, Marina; Čipak Gašparović, Ana; Milković, Lidija; Tartaro Bujak, Ivana; Mihaljević, Branka; Žarković, Neven; Žarković, Kamelija

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Cindrić, M., Čipak Gašparović, A., Milković, L., Tartaro Bujak, I., Mihaljević, B., Žarković, N. & Žarković, K. (2022). 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes. International Journal of Molecular Sciences, 23. (22). doi: 10.3390/ijms232214373

Cindrić, Marina, et al. "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes." International Journal of Molecular Sciences, vol. 23, no. 22, 2022. https://doi.org/10.3390/ijms232214373

Cindrić, Marina, Ana Čipak Gašparović, Lidija Milković, Ivana Tartaro Bujak, Branka Mihaljević, Neven Žarković and Kamelija Žarković. "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes." International Journal of Molecular Sciences 23, no. 22 (2022). https://doi.org/10.3390/ijms232214373

Cindrić, M., et al. (2022) '4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes', International Journal of Molecular Sciences, 23(22). doi: 10.3390/ijms232214373

Cindrić M, Čipak Gašparović A, Milković L, Tartaro Bujak I, Mihaljević B, Žarković N, and sur.. 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes. International Journal of Molecular Sciences [Internet]. 2022 November 19 [cited 2024 May 20];23(22). doi: 10.3390/ijms232214373

M. Cindrić, et al., "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes", International Journal of Molecular Sciences, vol. 23, no. 22, November 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:170700. [Accessed: 20 May 2024]

Metadata
Title (english)4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
AuthorMarina Cindrić
AuthorAna Čipak Gašparović
AuthorLidija Milković
AuthorIvana Tartaro Bujak
AuthorBranka Mihaljević
AuthorNeven Žarković
AuthorKamelija Žarković
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Abstract (english)
Blood brain barrier (BBB) is a dynamic interface responsible for proper functioning of brain, but also a major obstacle for effective treatment of neurological diseases. Increased levels of free radicals, in high ferrous and high lipid content surrounding, induce lipid peroxidation, leading to production of 4-hydroxynonenal (HNE). HNE modifies all key proteins responsible for proper brain functioning thus playing a major role in the onset of neurological diseases. To investigate HNE effects on BBB permeability, we developed two in vitro BBB models–‘physiological’ and ‘pathological’. The latter mimicked HNE modified extracellular matrix under oxidative stress conditions in brain pathologies. We showed that exogenous HNE induce activation of antioxidative defense systems by increasing catalase activity and glutathione content as well as reducing lipid peroxide levels in endothelial cells and astrocytes of ‘physiological’ model. While in ‘pathological’ model, exogenous HNE further increased lipid peroxidation levels of endothelial cells and astrocytes, followed by increase in Nrf2 and glutathione levels in endothelial cells. At lipid composition level, HNE caused increase in ω3 polyunsaturated fatty acid (PUFA) level in endothelial cells, followed by decrease in ω3 PUFA level and increase in monounsaturated fatty acid level in astrocytes. Using these models, we showed for the first time that HNE in ‘pathological’ model can reduce BBB permeability.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 23, no. 22, 14373
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms232214373
URN:NBNurn:nbn:hr:105:170700
Publication2022-11-19
ProjectNumber: 108-0000000-0028
Title (croatian): Oksidacijski stres i tumori središnjeg živčanog sustava
Title (english): Oxidative stress and central nervous system tumors
Leader: Kamelija Žarković
Jurisdiction: Croatia
Funding stream: ZP
ProjectNumber: 098-0982464-2519
Title (croatian): Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji
Title (english): Lipids, free radicals and their messengers in integrative oncology
Leader: Neven Žarković
Jurisdiction: Croatia
Funding stream: ZP
Document URLhttps://www.mdpi.com/1422-0067/23/22/14373
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-06-13 11:02:43