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Scientific paper - Original scientific paper
The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage
Brain Sciences, 13 (2023), 6; 910. https://doi.org/10.3390/brainsci13060910
Jagečić, Denis; Petrović, Dražen Juraj; Šimunić, Iva; Isaković, Jasmina; Mitrečić, Dinko

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Jagečić, D., Petrović, D. J., Šimunić, I., Isaković, J. & Mitrečić, D. (2023). The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage. Brain Sciences, 13. (6). doi: 10.3390/brainsci13060910

Jagečić, Denis, et al. "The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage." Brain Sciences, vol. 13, no. 6, 2023. https://doi.org/10.3390/brainsci13060910

Jagečić, Denis, Dražen Juraj Petrović, Iva Šimunić, Jasmina Isaković and Dinko Mitrečić. "The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage." Brain Sciences 13, no. 6 (2023). https://doi.org/10.3390/brainsci13060910

Jagečić, D., et al. (2023) 'The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage', Brain Sciences, 13(6). doi: 10.3390/brainsci13060910

Jagečić D, Petrović DJ, Šimunić I, Isaković J, Mitrečić D. The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage. Brain Sciences [Internet]. 2023 June 04 [cited 2024 November 26];13(6). doi: 10.3390/brainsci13060910

D. Jagečić, D. J. Petrović, I. Šimunić, J. Isaković and D. Mitrečić, "The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage", Brain Sciences, vol. 13, no. 6, June 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:578498. [Accessed: 26 November 2024]

Metadata
Title (english)The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells’ Differentiation Stage
AuthorDenis Jagečić
AuthorDražen Juraj Petrović
AuthorIva Šimunić
AuthorJasmina Isaković
AuthorDinko Mitrečić
Author's institutionUniversity of Zagreb
School of Medicine
(Croatian Institiute for Brain Research)
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract (english)
Perinatal brain damage, one of the most common causes of lifelong impairment, is predominantly caused by a lack of oxygen and glucose during early development. These conditions, in turn, affect cells of the nervous tissue through various stages of their maturation. To quantify the influence of these factors on cell differentiation and mitochondrial parameters, we exposed neural cell precursors to oxygen and glucose deprivation (OGD) during three stages of their differentiation: day 1, day 7, and day 14 (D1, D7, and D14, respectively). The obtained results show that OGD slows down cellular differentiation and causes cell death. Regardless of the level of cell maturity, the overall area of the mitochondria, their length, and the branching of their filaments decreased uniformly when exposed to OGD-related stress. Moreover, the cells in all stages of differentiation exhibited an increase in ROS production, hyperpolarization of the mitochondrial membrane, and autophagy. Interestingly, day 7 was the only stage in which a significant increase in mitochondrial fission, along with measurable instances of mitophagy, were detected. Taken together, the results of this study suggest that, apart from common reactions to a sudden lack of oxygen and glucose, cells in specific stages of neural differentiation can also exhibit increased preferences for mitochondrial fission and mitophagy. Such findings could play a role in guiding the future development of novel therapeutic approaches targeting perinatal brain damage during specific stages of nervous system development.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleBrain Sciences
Numberingvol. 13, no. 6, 910
e-ISSN2076-3425
DOIhttps://doi.org/10.3390/brainsci13060910
URN:NBNurn:nbn:hr:105:578498
Publication2023-06-04
ProjectNumber: IP-2016-06-9451
Title (croatian): Matične stanice usne šupljine čovjeka za liječenje ishemijske bolesti mozga
Acronym: ORASTEM
Leader: Dinko Mitrečić
Jurisdiction: Croatia
Funding stream: IP
Document URLhttps://www.mdpi.com/2076-3425/13/6/910
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2023-09-25 08:01:17