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master's thesis
Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica
Zagreb: University of Zagreb, School of Medicine, 2023. urn:nbn:hr:105:194065
Frančić, Manuela
University of Zagreb
School of Medicine
Department of Surgery

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Frančić, M. (2023). Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:194065

Frančić, Manuela. "Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica." Master's thesis, University of Zagreb, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:105:194065

Frančić, Manuela. "Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica." Master's thesis, University of Zagreb, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:105:194065

Frančić, M. (2023). 'Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica', Master's thesis, University of Zagreb, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:194065

Frančić M. Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2023 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:194065

M. Frančić, "Toksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:105:194065

Metadata
TitleToksikološka procjena nanočestica zlata u ulozi nosača L-Dope na in vitro modelu diferenciranih SH-SY5Y stanica
Title (english)Toxicological assessment of gold nanoparticles in the role od L-Dope carriers in the in vitro model of differentiated SH-SY5Y cells
AuthorManuela Frančić
MentorKrešimir Rotim (mentor)
Committee memberDavor Mijatović (predsjednik povjerenstva)
Committee memberAnko Antabak (član povjerenstva)
Committee memberKrešimir Rotim (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Surgery)
Zagreb
Defense date and country2023-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Surgery
Abstract
Primjena nanočestica zlata za dostavu L-Dope mogla bi se pokazati kao inovativna terapija Parkinsonove bolesti. No, prije ispitivanja njihove učinkovitosti, važno je utvrditi kompatibilnost s biološkim sustavima. Na modelu stanica sličnih dopaminergičkim neuronima ispitivan je utjecaj AuNP na vijabilnost i oksidativni stres, kao i moguće zaštitno djelovanje. Model stanica sličnih dopaminergičkim neuronima dobiven je RA/TPA diferencijacijom SH-SY5Y stanica što je potvrđeno qPCR-om i imunocitokemijom. Unos AuNP u stance određen je imunocitokemijom i protočnom citometrijom kojom su analizirane i stanična vijabilnost i apoptoza. Oksidativni stres istražen je mjerenjem razine reaktivnih kisikovih vrsta, koncentracije glutationa i potencijala mitohondrijske membrane.
Abstract (english)
The use of gold nanoparticles to deliver L-Dopa could prove to be an innovative therapy for Parkinson’s disease. But before examining their effectiveness, it is important to determine compatibility with biological systems. The effect of AuNP on viability and oxidative stress, as well as possible protective effects, was investigated in a cell model of dopaminergic neuron-like cells. A cell model similar to dopaminergic neurons was obtained by RA / TPA differentiation of SH-SY5Y cells as confirmed by qPCR and immunocytochemistry. The uptake of AuNP into cells was determined by immunocytochemistry and flow cytometry which analyzed both cell viability and apoptosis. Oxidative stress was investigated by measuring the level of reactive oxygen species, glutathione concentration, and mitochondrial membrane potential.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:194065
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-10-24 09:57:28