prikaz prve stranice dokumenta In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima
  Download
PDF 849.29 KB
master's thesis
In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima
Zagreb: University of Zagreb, School of Medicine, 2023. urn:nbn:hr:105:909438
Škripek, Željko
University of Zagreb
School of Medicine
Department of Medical Biology

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Škripek, Ž. (2023). In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:909438

Škripek, Željko. "In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima." Master's thesis, University of Zagreb, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:105:909438

Škripek, Željko. "In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima." Master's thesis, University of Zagreb, School of Medicine, 2023. https://urn.nsk.hr/urn:nbn:hr:105:909438

Škripek, Ž. (2023). 'In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima', Master's thesis, University of Zagreb, School of Medicine, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:909438

Škripek Ž. In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:909438

Ž. Škripek, "In silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:105:909438

Metadata
TitleIn silico analiza molekularnih biljega epitelno-mezenhimske tranzicije u gliomima
Title (english)In silico analysis of molecular markers of epithelial to mesenchymal transition in gliomas
AuthorŽeljko Škripek
MentorNives Pećina-Šlaus (mentor)
Committee memberAnja Kafka (predsjednik povjerenstva)
Committee memberLjiljana Šerman (član povjerenstva)
Committee memberNives Pećina-Šlaus (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Medical Biology)
Zagreb
Defense date and country2023-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
CILJ: Prikupiti podatke o mutacijama, amplifikacijama i delecijama gena uključenih u EMT te pridružiti učestalost genetičkih promjena s gliomima nižih (LGG) i viših gradusa (HGG). MATERIJALI I METODE: Retrospektivno prikupljeni podaci 13 odabranih gena u gliomima različitih patohistoloških tipova i gradusa korištenjem podataka u bazi cBioPortal. Analiza je uključivala mutacije pojedinog gena, promjene u broju kopija (CNA - copy number alteration) te razine transkripta (mRNA). REZULTATI: U istraživanju su obrađeni podaci od 3497 uzoraka. Samo glioblastomi i difuzni gliomi imaju promjene na svih 13 analiziranih gena. Anaplastični oligodendrogliom i anaplastični astrocitom imaju promjene u 46,15% analiziranih gena, oligodendrogliom u 23,08%, a oligoastrocitom u 15,38% analiziranih gena. Gen NOTCH1 bio je statistički značajno češće promijenjen u odnosu na gene CDH1, CTNNB1 i ZEB1 (p < 0,05). ZAKLJUČAK: Iz analize zaključujemo da su geni sudionici EMT promijenjeni u gliomima različitih gradusa. Najčešće mutirani i amplificirani bili su geni NOTCH1 i SOX2. Dodatna istraživanja i validacija rezultata dobivenih iz cBioPortal baze potrebna su kako bi potvrdila spoznaje koje je ovaj rad donio.
Abstract (english)
OBJECTIVES: Collect data of mutations, amplifications and deletions of genes involved in EMT and associate the frequency of genetic changes with low-grade (LGG) and high-grade (HGG) gliomas. MATERIALS AND METHODS: Retrospectively collected data of 13 selected genes in gliomas of different pathohistological types and grades using data in the cBioPortal database. The analysis included mutations of individual genes, changes in the copy number alteration (CNA) and transcript levels (mRNA). RESULTS: The research processed data from 3497 samples. Only glioblastoma and diffuse glioma have changes in all 13 analyzed genes. Anaplastic oligodendroglioma and anaplastic astrocytoma have changes in 46.15% of the analyzed genes, oligodendroglioma in 23.08% and oligoastrocytoma have changes in 15.38% of the analyzed genes. NOTCH1 gene was statistically more frequently changed compared to the CDH1, CTNNB1 and ZEB1 genes (p < 0,05). CONCLUSION: From the analysis, we conclude that genes involved in EMT are changed in gliomas of different grades. The most frequently mutated and amplified genes were NOTCH1 and SOX2. Additional research and validation of the results obtained from the cBioPortal database are needed to confirm the knowledge this paper has brought.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:909438
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-11-02 10:36:12