Antineoplastic drugs, surgery and radiotherapy are the main methods of treating malignant diseases. According to the definitions of the National Institute for Occupational Safety and Health (NIOSH) and the quality standards of the European Society of Oncology Pharmacy (ESOP), cytostatics form a group of drugs that are classified as hazardous drugs. Pharmaceutical workers and nurses are the most exposed group of health workers to antineoplastic drugs. Although the exposure is not as direct as in patients, due to frequent exposure they may be affected by their effects. A survey was conducted among nurses employed at the hematology and oncology departments at the University Hospital Centre Zagreb. Aim of the research is to examine the incidence of health problems that nurses associate with occupational exposure to antineoplastic drugs. For the purposes of the research, a survey was compiled with the help of a mentor, and it was conducted during the month of May 2022. Out of a possible 210 respondents, 130 participated in the survey (response rate of 61.9%). The obtained results showed that the respondents regularly go for periodical health examinations, participate in education and they are familiar with guidelines on the safe handling of antineoplastic drugs. Most respondents use personal protective equipment while working with antineoplastic drugs. Cyclophosphamide, methotrexate, etoposide, doxorubicin and imatinib are the most frequently used antineoplastic drugs at the respondents' workplace. Out of 130 respondents who participated in survey, 10 respondents associate their health problems with professional exposure to antineoplastic drugs, but 70% of them did not have their complaints medically verified.