prikaz prve stranice dokumenta VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia
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Scientific paper - Original scientific paper
VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia
Scandinavian Journal of Infectious Diseases, 42 (2010), 3; 193-197. https://doi.org/10.3109/00365540903426582
Bošnjak, Zrinka; Bedenić, Branka; Mazzariol, Annarita; Jarža-Davila, Neda; Šuto, Sandra; Kalenić, Smilja

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Bošnjak, Z., Bedenić, B., Mazzariol, A., Jarža Davila, N., Šuto, S. & Kalenić, S. (2010). VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia. Scandinavian Journal of Infectious Diseases, 42. (3), 193-197. doi: 10.3109/00365540903426582

Bošnjak, Zrinka, et al. "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia." Scandinavian Journal of Infectious Diseases, vol. 42, no. 3, 2010, pp. 193-197. https://doi.org/10.3109/00365540903426582

Bošnjak, Zrinka, Branka Bedenić, Annarita Mazzariol, Neda Jarža Davila, Sandra Šuto and Smilja Kalenić. "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia." Scandinavian Journal of Infectious Diseases 42, no. 3 (2010): 193-197. https://doi.org/10.3109/00365540903426582

Bošnjak, Z., et al. (2010) 'VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia', Scandinavian Journal of Infectious Diseases, 42(3), pp. 193-197. doi: 10.3109/00365540903426582

Bošnjak Z, Bedenić B, Mazzariol A, Jarža Davila N, Šuto S, Kalenić S. VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia. Scandinavian Journal of Infectious Diseases [Internet]. 2010 March [cited 2024 April 27];42(3):193-197. doi: 10.3109/00365540903426582

Z. Bošnjak, B. Bedenić, A. Mazzariol, N. Jarža Davila, S. Šuto and S. Kalenić, "VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia", Scandinavian Journal of Infectious Diseases, vol. 42, no. 3, pp. 193-197, March 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:875221. [Accessed: 27 April 2024]

Metadata
Title (english)VIM-2 beta-lactamase in Pseudomonas aeruginosa isolates from Zagreb, Croatia
AuthorZrinka Bošnjak
AuthorBranka Bedenić
AuthorAnnarita Mazzariol
AuthorNeda Jarža-Davila
AuthorSandra Šuto
AuthorSmilja Kalenić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
The aim of this investigation was to characterize metallo-beta-lactamases (MBLs) in Pseudomonas aeruginosa isolates from Zagreb, Croatia. One hundred P. aeruginosa isolates with reduced susceptibility to either imipenem or meropenem were tested for the production of MBLs by MBL-Etest. The susceptibility to a wide range of antibiotics was determined by broth microdilution method. The presence of bla(MBL) genes was detected by polymerase chain reaction (PCR). Hydrolysis of 0.1 mM imipenem by crude enzyme preparations of beta-lactamases was monitored by UV spectrophotometer. Outer membrane proteins were prepared and analysed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Six out of 100 isolates were positive for MBLs by Etest. All strains were resistant to gentamicin, ceftazidime and cefotaxime, and all except 1 were resistant to imipenem. Six strains positive for MBLs by Etest were identified as VIM MBL-producers by PCR. Sequencing of bla(VIM) genes revealed the production of VIM-2 beta-lactamase in all 6 strains. This investigation proved the occurrence of VIM-2 beta-lactamase among P. aeruginosa strains from Zagreb, Croatia. VIM-2 beta-lactamase with similar properties has previously been described in another region of Croatia and in Italy, France, Spain, Greece, Taiwan and South Korea, suggesting that this type of enzyme is widespread in the Mediterranean region of Europe and in the Far East.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleScandinavian Journal of Infectious Diseases
Numberingvol. 42, no. 3, pp. 193-197
p-ISSN0036-5548
DOIhttps://doi.org/10.3109/00365540903426582
URN:NBNurn:nbn:hr:105:875221
Publication2010-03
Document URLhttps://doi.org/10.3109/00365540903426582
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-27 19:07:19