prikaz prve stranice dokumenta Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children
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Scientific paper - Original scientific paper
Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children
Wiener Klinische Wochenschrift, 124 (2012), 15-16; 504-515. https://doi.org/10.1007/s00508-012-0210-5
Bedenić, Branka; Vraneš, Jasmina; Hofmann-Thiel, Sabine; Tonkić, Marija; Novak, Anita; Bučević-Popović, Viljemka; Hoffmann, Harald

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Bedenić, B., Vraneš, J., Hofmann Thiel, S., Tonkić, M., Novak, A., Bučević Popović, V. & Hoffmann, H. (2012). Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children. Wiener Klinische Wochenschrift, 124. (15-16), 504-515. doi: 10.1007/s00508-012-0210-5

Bedenić, Branka, et al. "Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children." Wiener Klinische Wochenschrift, vol. 124, no. 15-16, 2012, pp. 504-515. https://doi.org/10.1007/s00508-012-0210-5

Bedenić, Branka, Jasmina Vraneš, Sabine Hofmann Thiel, Marija Tonkić, Anita Novak, Viljemka Bučević Popović and Harald Hoffmann. "Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children." Wiener Klinische Wochenschrift 124, no. 15-16 (2012): 504-515. https://doi.org/10.1007/s00508-012-0210-5

Bedenić, B., et al. (2012) 'Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children', Wiener Klinische Wochenschrift, 124(15-16), pp. 504-515. doi: 10.1007/s00508-012-0210-5

Bedenić B, Vraneš J, Hofmann Thiel S, Tonkić M, Novak A, Bučević Popović V, and sur.. Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children. Wiener Klinische Wochenschrift [Internet]. 2012 August [cited 2024 December 25];124(15-16):504-515. doi: 10.1007/s00508-012-0210-5

B. Bedenić, et al., "Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children", Wiener Klinische Wochenschrift, vol. 124, no. 15-16, pp. 504-515, August 2012. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:007336. [Accessed: 25 December 2024]

Metadata
Title (english)Characterization of the extended-spectrum β-lactamases and determination of the virulence factors of uropathogenic Escherichia coli strains isolated from children
AuthorBranka Bedenić
AuthorJasmina Vraneš
AuthorSabine Hofmann-Thiel
AuthorMarija Tonkić
AuthorAnita Novak
AuthorViljemka Bučević-Popović
AuthorHarald Hoffmann
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
BACKGROUND AND AIM: The aim of the study was to characterize ESBL-producing uropathogenic Escherichia coli (UPEC) strains isolated in children. That included the investigation of virulence factors and the analysis of the types of β-lactamases at the molecular genetic level. ----- MATERIAL AND METHODS: During the 2-year study period, 77 ESBL-producing E. coli strains were recovered from urine samples of febrile children with significant bacteriuria hospitalized at one Croatian hospital. Susceptibility of isolates to bactericidal serum activity was tested by Shiller and Hatch method, while adhesin expression was determined by agglutination methods. Characterization of ESBLs was performed by PCR with specific primers for ESBLs and by sequencing of bla (ESBL) genes. Genotyping of the E. coli isolates was performed by pulsed-field gel electrophoresis (PFGE). ----- RESULTS: Twenty-seven (35.1 %) and 50 (64.9 %) ESBL-producing UPEC strains were isolated in neonates and infants, respectively. Of 70 strains investigated for the presence of virulence factors, adhesins were detected in 48.6 % strains (8.6 % in the neonate and 40 % in the infants group) giving a statistically significant difference in adhesin expression between the two groups (p < 0.01). Hemolysin was produced by 84.3 %, whereas 70 % of strains were serum-resistant. The bla (TEM) gene was detected in 22 (28 %) and bla (SHV) gene in 57 strains (74 %), whereas bla (CTX-M) gene was detected in only two isolates (2.5%). In ten isolates, bla (TEM) and bla (SHV) were simultaneously detected. Sequencing of bla (SHV) genes revealed that SHV-5 β-lactamase was by far the most prevalent and was found in 51 strains (66 %). The strains were clonally related as demonstrated by PFGE and assigned into ten clusters. ----- CONCLUSIONS: Infection control measures should be employed and the consumption of expanded-spectrum cephalosporins in the hospital should be restricted.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleWiener Klinische Wochenschrift
Numberingvol. 124, no. 15-16, pp. 504-515
p-ISSN0043-5325
DOIhttps://doi.org/10.1007/s00508-012-0210-5
URN:NBNurn:nbn:hr:105:007336
Publication2012-08
Document URLhttps://doi.org/10.1007/s00508-012-0210-5
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-27 19:37:53