prikaz prve stranice dokumenta Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
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Scientific paper - Original scientific paper
Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
Croatian Medical Journal, 54 (2013), 1; 3-11. https://doi.org/10.3325/cmj.2013.54.3
Dobrivojević, Marina; Bohaček, Ivan; Erjavec, Igor; Gorup, Dunja; Gajović, Srećko

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Dobrivojević, M., Bohaček, I., Erjavec, I., Gorup, D. & Gajović, S. (2013). Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents. Croatian Medical Journal, 54. (1), 3-11. doi: 10.3325/cmj.2013.54.3

Dobrivojević, Marina, et al. "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents." Croatian Medical Journal, vol. 54, no. 1, 2013, pp. 3-11. https://doi.org/10.3325/cmj.2013.54.3

Dobrivojević, Marina, Ivan Bohaček, Igor Erjavec, Dunja Gorup and Srećko Gajović. "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents." Croatian Medical Journal 54, no. 1 (2013): 3-11. https://doi.org/10.3325/cmj.2013.54.3

Dobrivojević, M., et al. (2013) 'Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents', Croatian Medical Journal, 54(1), pp. 3-11. doi: 10.3325/cmj.2013.54.3

Dobrivojević M, Bohaček I, Erjavec I, Gorup D, Gajović S. Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents. Croatian Medical Journal [Internet]. 2013 February [cited 2024 November 09];54(1):3-11. doi: 10.3325/cmj.2013.54.3

M. Dobrivojević, I. Bohaček, I. Erjavec, D. Gorup and S. Gajović, "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents", Croatian Medical Journal, vol. 54, no. 1, pp. 3-11, February 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:199163. [Accessed: 09 November 2024]

Metadata
Title (english)Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents
AuthorMarina Dobrivojević
AuthorIvan Bohaček
AuthorIgor Erjavec
AuthorDunja Gorup
AuthorSrećko Gajović
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
AIM: To explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse brain ischemic lesions after middle cerebral artery occlusion (MCAO). ----- METHODS: Isolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned using microCT scanner. To verify whether ex-vivo microCT brain images can be used to characterize ischemic lesions, they were compared to Nissl stained serial histological sections of the same brains. To verify if brains immersed in RCA may be used afterwards for other methods, subsequent immunofluorescent labeling with anti-NeuN was performed. ----- RESULTS: Nonionic RCA showed better gray to white matter contrast in the brain, and therefore was selected for further studies. MicroCT measurement of ischemic lesion size and cerebral edema significantly correlated with the values determined by Nissl staining (ischemic lesion size: P=0.0005; cerebral edema: P=0.0002). Brain immersion in nonionic RCA did not affect subsequent immunofluorescent analysis and NeuN immunoreactivity. ----- CONCLUSION: MicroCT method was proven to be suitable for delineation of the ischemic lesion from the non-infarcted tissue, and quantification of lesion volume and cerebral edema.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatian Medical Journal
Numberingvol. 54, no. 1, pp. 3-11
p-ISSN0353-9504
DOIhttps://doi.org/10.3325/cmj.2013.54.3
URN:NBNurn:nbn:hr:105:199163
Publication2013-02
Document URLhttps://doi.org/10.3325/cmj.2013.54.3
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-27 19:51:48