prikaz prve stranice dokumenta Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
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Scientific paper - Original scientific paper
Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
Croatian Medical Journal, 54 (2013), 5; 489-495. https://doi.org/10.3325/cmj.2013.54.489
Mužić, Vedrana; Katušić Bojanac, Ana; Jurić-Lekić, Gordana; Himelreich, Marta; Tupek, Katarina; Šerman, Ljiljana; Marn, Nina; Sinčić, Nino; Vlahović, Maja; Bulić-Jakuš, Floriana

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Mužić, V., Katušić Bojanac, A., Jurić Lekić, G., Himelreich, M., Tupek, K., Šerman, Lj. ... Bulić Jakuš, F. (2013). Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro. Croatian Medical Journal, 54. (5), 489-495. doi: 10.3325/cmj.2013.54.489

Mužić, Vedrana, et al. "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro." Croatian Medical Journal, vol. 54, no. 5, 2013, pp. 489-495. https://doi.org/10.3325/cmj.2013.54.489

Mužić, Vedrana, Ana Katušić Bojanac, Gordana Jurić Lekić, Marta Himelreich, Katarina Tupek, Ljiljana Šerman, Nina Marn, Nino Sinčić, Maja Vlahović and Floriana Bulić Jakuš. "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro." Croatian Medical Journal 54, no. 5 (2013): 489-495. https://doi.org/10.3325/cmj.2013.54.489

Mužić, V., et al. (2013) 'Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro', Croatian Medical Journal, 54(5), pp. 489-495. doi: 10.3325/cmj.2013.54.489

Mužić V, Katušić Bojanac A, Jurić Lekić G, Himelreich M, Tupek K, Šerman Lj, and sur.. Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro. Croatian Medical Journal [Internet]. 2013 October 28 [cited 2024 May 03];54(5):489-495. doi: 10.3325/cmj.2013.54.489

V. Mužić, et al., "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro", Croatian Medical Journal, vol. 54, no. 5, pp. 489-495, October 2013. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:251611. [Accessed: 03 May 2024]

Metadata
Title (english)Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
AuthorVedrana Mužić
AuthorAna Katušić Bojanac
AuthorGordana Jurić-Lekić
AuthorMarta Himelreich
AuthorKatarina Tupek
AuthorLjiljana Šerman
AuthorNina Marn
AuthorNino Sinčić
AuthorMaja Vlahović
AuthorFloriana Bulić-Jakuš
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
AIM: To establish an organotypic in vitro model of limb bud development to verify whether epigenetic drug and teratogen 5-azacytidine (5azaC) has an effect on limb buds independent of its effects on the placenta. ----- METHODS: Fischer strain rat fore- and hindlimb buds were microsurgically isolated from 13 days old embryos and cultivated in vitro for two weeks at the air-liquid interface in Eagle's minimum essential medium (MEM) with 50% rat serum. 30 μmol of 5azaC was added to the fresh medium. Overall growth was measured by an ocular micrometer. Routine histology, immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), and stereological quantification of PCNA expression were performed. ----- RESULTS: At four time points, significantly lower overall growth was detected for fore- and hindlimb bud explants cultivated with 5azaC in comparison to controls. After the culture period, numerical density of the PCNA signal for both types of limb buds was lower than for controls (P<0.001). Limb buds were initially covered by immature epithelium and contained mesenchyme, myotubes, single hemangioblasts, hemangioblast aggregates, blood islands, and capillaries. Regardless of the treatment, cartilage and epidermis differentiated, but cells and structures typical for vasculogenesis disappeared. ----- CONCLUSION: Our findings, obtained outside of the maternal organism, stress the importance of compromised cell proliferation for 5azaC impact on limb buds. This investigation points to the necessity to establish alternatives to in vivo research on animals using teratogenic agents.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatian Medical Journal
Numberingvol. 54, no. 5, pp. 489-495
p-ISSN0353-9504
DOIhttps://doi.org/10.3325/cmj.2013.54.489
URN:NBNurn:nbn:hr:105:251611
Publication2013-10-28
Document URLhttps://doi.org/10.3325/cmj.2013.54.489
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-27 20:07:11