prikaz prve stranice dokumenta Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
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Scientific paper - Original scientific paper
Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
International Journal of Molecular Sciences, 15 (2014), 6; 10635-10651. https://doi.org/10.3390/ijms150610635
Kafka, Anja; Tomas, Davor; Beroš, Vili; Pećina, Hrvoje Ivan; Zeljko, Martina; Pećina-Šlaus, Nives

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Kafka, A., Tomas, D., Beroš, V., Pećina, H. I., Zeljko, M. & Pećina Šlaus, N. (2014). Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin. International Journal of Molecular Sciences, 15. (6), 10635-10651. doi: 10.3390/ijms150610635

Kafka, Anja, et al. "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin." International Journal of Molecular Sciences, vol. 15, no. 6, 2014, pp. 10635-10651. https://doi.org/10.3390/ijms150610635

Kafka, Anja, Davor Tomas, Vili Beroš, Hrvoje Ivan Pećina, Martina Zeljko and Nives Pećina Šlaus. "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin." International Journal of Molecular Sciences 15, no. 6 (2014): 10635-10651. https://doi.org/10.3390/ijms150610635

Kafka, A., et al. (2014) 'Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin', International Journal of Molecular Sciences, 15(6), pp. 10635-10651. doi: 10.3390/ijms150610635

Kafka A, Tomas D, Beroš V, Pećina HI, Zeljko M, Pećina Šlaus N. Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin. International Journal of Molecular Sciences [Internet]. 2014 June 13 [cited 2024 November 09];15(6):10635-10651. doi: 10.3390/ijms150610635

A. Kafka, D. Tomas, V. Beroš, H. I. Pećina, M. Zeljko and N. Pećina Šlaus, "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin", International Journal of Molecular Sciences, vol. 15, no. 6, pp. 10635-10651, June 2014. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:707303. [Accessed: 09 November 2024]

Metadata
Title (english)Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
AuthorAnja Kafka
AuthorDavor Tomas
AuthorVili Beroš
AuthorHrvoje Ivan Pećina
AuthorMartina Zeljko
AuthorNives Pećina-Šlaus
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
The susceptibility of brain to secondary formation from lung cancer primaries is a well-known phenomenon. In contrast, the molecular basis for invasion and metastasis to the brain is largely unknown. In the present study, 31 brain metastases that originated from primary lung carcinomas were analyzed regarding over expression of Dishevelled-1 (DVL1), Dishevelled-3 (DVL3), E-cadherin (CDH1) and beta-catenin (CTNNB1). Protein expressions and localizations were analyzed by immunohistochemistry. Genetic alterations of E-cadherin were tested by polymerase chain reaction (PCR)/loss of heterozygosity (LOH). Heteroduplex was used to investigate mutations in beta-catenin. DVL1 and DVL3 showed over expression in brain metastasis in 87.1% and 90.3% of samples respectively. Nuclear staining was observed in 54.8% of cases for DVL1 and 53.3% for DVL3. The main effector of the Wnt signaling, beta-catenin, was up-regulated in 56%, and transferred to the nucleus in 36% of metastases. When DVL1 and DVL3 were up-regulated the number of cases with nuclear beta-catenin significantly increased (p=0.0001). Down-regulation of E-cadherin was observed in 80% of samples. Genetic analysis showed 36% of samples with LOH of the CDH1. In comparison to other lung cancer pathologies, the diagnoses adenocarcinoma and small cell lung cancer (SCLC) were significantly associated to CDH1 LOH (p=0.001). Microsatellite instability was detected in one metastasis from adenocarcinoma. Exon 3 of beta-catenin was not targeted. Altered expression of Dishevelled-1, Dishevelled-3, E-cadherin and beta-catenin were present in brain metastases which indicates that Wnt signaling is important and may contribute to better understanding of genetic profile conditioning lung cancer metastasis to the brain.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 15, no. 6, pp. 10635-10651
p-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms150610635
URN:NBNurn:nbn:hr:105:707303
Publication2014-06-13
Document URLhttp://doi.org/10.3390/ijms150610635
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:07:05