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master's thesis
Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka
Zagreb: University of Zagreb, School of Medicine, 2015. urn:nbn:hr:105:352702
Radonić, Vedran
University of Zagreb
School of Medicine
Department of Physics and Biophysics

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Radonić, V. (2015). Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:352702

Radonić, Vedran. "Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka." Master's thesis, University of Zagreb, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:105:352702

Radonić, Vedran. "Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka." Master's thesis, University of Zagreb, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:105:352702

Radonić, V. (2015). 'Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka', Master's thesis, University of Zagreb, School of Medicine, accessed 26 April 2024, https://urn.nsk.hr/urn:nbn:hr:105:352702

Radonić V. Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2015 [cited 2024 April 26] Available at: https://urn.nsk.hr/urn:nbn:hr:105:352702

V. Radonić, "Procjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:105:352702

Metadata
TitleProcjena embriotoksičnosti valproata iz infracrvenih spektara metaboloma medija tijekom kulture štakorskog zametka
Title (english)Assessment of valproate embriotoxicity from infrared spectra of ebrio culture media metabolome
AuthorVedran Radonić
MentorMaja Balarin (mentor)
Committee memberSanja Dolanski-Babić (predsjednik povjerenstva)
Committee memberOzren Gamulin (član povjerenstva)
Committee memberMaja Balarin (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Physics and Biophysics)
Zagreb
Defense date and country2015-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uzorci medija u kojima su rasli štakorski zameci prikupljani su na Zavodu za biologiju i pohranjivani za daljnju analizu. FTIR spektroskopijom snimljeni su spektri metaboloma medija s i bez valproata u kojima su 14 dana rasli štakorski zameci. Spektri su analizirani statističkim metodama PCA i PC regresije. Pokazalo se da metoda FTIR spektroskopije može razlikovati metabolom medija kontrole u kojem su rasli štakorski zameci bez valproata od onog s valproatom. Pored toga utvrdili smo kako spektroskopska analiza metaboloma medija sadrži podatak o razlici u fazi rasta i razvoja zametaka kroz promatrani period vremena te smo pokazali da se iz spektara medija može odrediti koliko dugo su zameci rasli u kulturi. Rezultati ovog rada ukazuju da FTIR spektroskopska analiza metaboloma medija može biti novi model za probir različitih embriotoksičnih i teratogenih supstanci.
Abstract (english)
Samples of the media in which rat embryos grew, were collected at the Department of Biology and stored for further analysis. Using FTIR spectroscopy the spectra of media metabolome in which rat embryos grew for 14 days, with and without valproate, were recorded. The spectra were analyzed by statistical methods of PCA and PC regression. It was shown that the method of FTIR spectroscopy can distinguish media metabolome between control group in which rat embryos grew without valproate and the one in which they grew with valproate. We have established that the spectroscopic analysis of the metabolome media contains information about the difference in the growth and development of embryos through the monitored period of time. Using PCA analysis we have shown that from metabolome spectra we can determine for how long the embryos grow in culture. These results suggest that FTIR spectroscopic analysis of the media metabolome can be a new model for the screening of different embryo toxic and teratogenic substances.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:352702
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2016-08-09 07:04:52