prikaz prve stranice dokumenta Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer
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Scientific paper - Original scientific paper
Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer
World Journal of Surgical Oncology, 13 (2015); 241. https://doi.org/10.1186/s12957-015-0667-6
Matošević, Petar; Klepac-Pulanić, Tajana; Kinda, Emil; Augustin, Goran; Brčić, Iva; Jakić-Razumović, Jasminka

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Matošević, P., Klepac Pulanić, T., Kinda, E., Augustin, G., Brčić, I. & Jakić Razumović, J. (2015). Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer. World Journal of Surgical Oncology, 13., 241. doi: 10.1186/s12957-015-0667-6

Matošević, Petar, et al. "Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer." World Journal of Surgical Oncology, vol. 13, 2015, p. 241. https://doi.org/10.1186/s12957-015-0667-6

Matošević, Petar, Tajana Klepac Pulanić, Emil Kinda, Goran Augustin, Iva Brčić and Jasminka Jakić Razumović. "Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer." World Journal of Surgical Oncology 13 (2015): 241. https://doi.org/10.1186/s12957-015-0667-6

Matošević, P., et al. (2015) 'Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer', World Journal of Surgical Oncology, 13, p. 241. doi: 10.1186/s12957-015-0667-6

Matošević P, Klepac Pulanić T, Kinda E, Augustin G, Brčić I, Jakić Razumović J. Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer. World Journal of Surgical Oncology [Internet]. 2015 August 07 [cited 2024 May 06];13:241. doi: 10.1186/s12957-015-0667-6

P. Matošević, T. Klepac Pulanić, E. Kinda, G. Augustin, I. Brčić and J. Jakić Razumović, "Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer", World Journal of Surgical Oncology, vol. 13, p. 241, August 2015. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:053133. [Accessed: 06 May 2024]

Metadata
Title (english)Immunohistochemical expression of 8-oxo-7,8-dihydro-2′-deoxyguanosine in cytoplasm of tumour and adjacent normal mucosa cells in patients with colorectal cancer
AuthorPetar Matošević
AuthorTajana Klepac-Pulanić
AuthorEmil Kinda
AuthorGoran Augustin
AuthorIva Brčić
AuthorJasminka Jakić-Razumović
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
BACKGROUND: The aim of this research was to study the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in tumour tissue samples of colorectal carcinoma based upon immunohistochemical detection and compare those results with patients' outcome. ----- METHODS: Tumour blocks of patients surgically treated for colorectal cancer were evaluated by 8-oxodG immunohistochemical staining. The expression was analysed in 500 tumour cells. The percentage of positive cells, as well as staining intensity, was recorded, and Allred score was calculated. For each patient, data of age, gender, tumour size and location, margin status, histologic grade, tumour stage, lymph node status, vascular invasion, overall survival, and therapy protocols were collected. Tumour grade was divided into two groups as low and high grade. ----- RESULTS: In this study, 146 consecutive patients with primary colorectal carcinoma were included. All data were available for 138 patients, and they were included in this research. There were 83 male and 55 female patients; the median age was 64 years (range 35-87 years). The results showed shorter 5- and 10-year survival in patients with 8-oxodG positive tumour cells (5-year survival, n=138, Mantel-Cox, chi-square 4.116, degree of freedom (df)=1, p<0.05; 10-year survival, n=134, Mantel-Cox, chi-square 4.374, df=1, p<0.05). The results showed a positive correlation between Allred score and high tumour grade (two-tailed Spearman's ρ 0.184; p<0.05), as well as with non-polypoid tumour growth (two-tailed Spearman's ρ 0.198; p<0.05). There was no significant difference of 8-oxodG expression related to age, sex, blood group, size and tumour site, distance from the edge of the resected tumour margin, lymph nodes involvement, and vascular invasion. ----- CONCLUSIONS: In this study, the positive correlation between 8-oxodG presence in the tumour cells, worse clinical outcome, higher tumour grade, and flat morphology was found.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleWorld Journal of Surgical Oncology
Numberingvol. 13, p. 241
p-ISSN1477-7819
DOIhttps://doi.org/10.1186/s12957-015-0667-6
URN:NBNurn:nbn:hr:105:053133
Publication2015-08-07
Document URLhttps://doi.org/10.1186/s12957-015-0667-6
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:40:00