prikaz prve stranice dokumenta Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis
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Scientific paper - Original scientific paper
Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis
Acta Informatica Medica, 24 (2016), 4; 248-252. https://doi.org/10.5455/aim.2016.24.248-252
Rebić, Velma; Budimir, Ana; Aljičević, Mufida; Bektaš, Sabaheta; Mahmutović Vranić, Sabina; Rebić, Damir

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Rebić, V., Budimir, A., Aljičević, M., Bektaš, S., Mahmutović Vranić, S. & Rebić, D. (2016). Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis. Acta Informatica Medica, 24. (4), 248-252. doi: 10.5455/aim.2016.24.248-252

Rebić, Velma, et al. "Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis." Acta Informatica Medica, vol. 24, no. 4, 2016, pp. 248-252. https://doi.org/10.5455/aim.2016.24.248-252

Rebić, Velma, Ana Budimir, Mufida Aljičević, Sabaheta Bektaš, Sabina Mahmutović Vranić and Damir Rebić. "Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis." Acta Informatica Medica 24, no. 4 (2016): 248-252. https://doi.org/10.5455/aim.2016.24.248-252

Rebić, V., et al. (2016) 'Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis', Acta Informatica Medica, 24(4), pp. 248-252. doi: 10.5455/aim.2016.24.248-252

Rebić V, Budimir A, Aljičević M, Bektaš S, Mahmutović Vranić S, Rebić D. Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis. Acta Informatica Medica [Internet]. 2016 July 18 [cited 2024 April 30];24(4):248-252. doi: 10.5455/aim.2016.24.248-252

V. Rebić, A. Budimir, M. Aljičević, S. Bektaš, S. Mahmutović Vranić and D. Rebić, "Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis", Acta Informatica Medica, vol. 24, no. 4, pp. 248-252, July 2016. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:216461. [Accessed: 30 April 2024]

Metadata
Title (english)Typing of methicillin resistant Staphylococcus aureus using DNA fingerprints by pulsed-field gel electrophoresis
AuthorVelma Rebić
AuthorAna Budimir
AuthorMufida Aljičević
AuthorSabaheta Bektaš
AuthorSabina Mahmutović Vranić
AuthorDamir Rebić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to analyze MRSA strains from the general population in Canton Sarajevo, B&H. ----- METHODS: Our investigation including either phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and Panton-Valentine leukocidin (PVL) detection. ----- RESULTS: Antimicrobial susceptibility: all MRSA isolates were resistant to the β-lactam antibiotics tested, and all isolates were susceptible trimethoprim sulphamethoxazole, rifampicin, fusidic acid, linezolid and vancomycin. Sixty-eight per cent of the MRSA isolates were resistant to erythromycin, 5% to clindamycin, 5% to gentamicin and 4% to ciprofloxacin. After the PFGE analysis, the isolates were grouped into five similarity groups: A-E. The largest number of isolates belonged to one of two groups: C: 60 (60%) and D: 27 (27%). In both groups C and D, SCCmec type IV was predominant (60% and 88, 8%, respectively). A total of 24% of the isolates had positive expression of PVL genes, while 76% showed a statistically significantly greater negative expression of PVL genes. ----- CONCLUSION: SCCmec type IV, together with the susceptibility profile and PFGE grouping, is considered to be typical of CA-MRSA.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleActa Informatica Medica
Numberingvol. 24, no. 4, pp. 248-252
p-ISSN0353-8109
DOIhttps://doi.org/10.5455/aim.2016.24.248-252
URN:NBNurn:nbn:hr:105:216461
Publication2016-07-18
Document URLhttps://doi.org/10.5455/aim.2016.24.248-252
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:51:32