prikaz prve stranice dokumenta Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
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Scientific paper - Original scientific paper
Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
Journal of Virological Methods, 135 (2006), 2; 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013
Kozić, Sanja; Vince, Adriana; Iščić Beš, Janja; Đaković Rode, Oktavija; Židovec Lepej, Snježana; Poljak, Mario; Bozic, Michael; Kessler, Harald H.

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Kozić, S., Vince, A., Iščić Beš, J., Đaković Rode, O., Židovec Lepej, S., Poljak, M. ... Kessler, H. H. (2006). Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients. Journal of Virological Methods, 135. (2), 263-268. doi: 10.1016/j.jviromet.2006.03.013

Kozić, Sanja, et al. "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients." Journal of Virological Methods, vol. 135, no. 2, 2006, pp. 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013

Kozić, Sanja, Adriana Vince, Janja Iščić Beš, Oktavija Đaković Rode, Snježana Židovec Lepej, Mario Poljak, Michael Bozic and Harald H. Kessler. "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients." Journal of Virological Methods 135, no. 2 (2006): 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013

Kozić, S., et al. (2006) 'Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients', Journal of Virological Methods, 135(2), pp. 263-268. doi: 10.1016/j.jviromet.2006.03.013

Kozić S, Vince A, Iščić Beš J, Đaković Rode O, Židovec Lepej S, Poljak M, and sur.. Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients. Journal of Virological Methods [Internet]. 2006 [cited 2024 May 03];135(2):263-268. doi: 10.1016/j.jviromet.2006.03.013

S. Kozić, et al., "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients", Journal of Virological Methods, vol. 135, no. 2, pp. 263-268, 2006. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:744800. [Accessed: 03 May 2024]

Metadata
Title (english)Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
AuthorSanja Kozić
AuthorAdriana Vince
AuthorJanja Iščić Beš
AuthorOktavija Đaković Rode
AuthorSnježana Židovec Lepej
AuthorMario Poljak
AuthorMichael Bozic
AuthorHarald H. Kessler
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
The aim of this study was to evaluate the performance of a molecular assay for quantitation of Epstein-Barr virus (EBV) DNA based on real-time PCR, and to determine EBV DNA levels in EDTA whole blood samples derived from different groups of patients. Following a manual DNA extraction protocol, real-time PCR was performed using the LightCycler EBV Quantification Kit, which demonstrated sufficient accuracy and linearity. Coefficients of variations were found to be between 6 and 42% and 5 and 34%, respectively, for inter-assay and intra-assay variations. In clinical specimens, EBV DNA was detected in all patients with acute EBV infection (n=34), in one of 25 adults with past EBV infection, in 16 out of 25 (64%) anti-HIV antibody positive persons, in 10 out of 25 (40%) solid organ transplant recipients, and in none of the 23 infants without history of EBV infection. When EBV DNA levels in positive specimens were compared between different groups, statistically significant differences were not found. The LightCycler EBV Quantification Kit was found to be useful for determination of EBV DNA levels in EDTA whole blood.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleJournal of Virological Methods
Numberingvol. 135, no. 2, pp. 263-268
p-ISSN0166-0934
DOIhttps://doi.org/10.1016/j.jviromet.2006.03.013
URN:NBNurn:nbn:hr:105:744800
Publication2006
Document URLhttps://doi.org/10.1016/j.jviromet.2006.03.013
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 08:59:12