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Scientific paper - Original scientific paper
Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry
International Orthopaedics, 31 (2007), 6; 743-751. https://doi.org/10.1007/s00264-007-0404-z
Grgurević, Lovorka; Maček, Boris; Đurđević, Dragan; Vukičević, Slobodan

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Grgurević, L., Maček, B., Đurđević, D. & Vukičević, S. (2007). Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry. International Orthopaedics, 31. (6), 743-751. doi: 10.1007/s00264-007-0404-z

Grgurević, Lovorka, et al. "Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry." International Orthopaedics, vol. 31, no. 6, 2007, pp. 743-751. https://doi.org/10.1007/s00264-007-0404-z

Grgurević, Lovorka, Boris Maček, Dragan Đurđević and Slobodan Vukičević. "Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry." International Orthopaedics 31, no. 6 (2007): 743-751. https://doi.org/10.1007/s00264-007-0404-z

Grgurević, L., et al. (2007) 'Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry', International Orthopaedics, 31(6), pp. 743-751. doi: 10.1007/s00264-007-0404-z

Grgurević L, Maček B, Đurđević D, Vukičević S. Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry. International Orthopaedics [Internet]. 2007 December [cited 2024 May 06];31(6):743-751. doi: 10.1007/s00264-007-0404-z

L. Grgurević, B. Maček, D. Đurđević and S. Vukičević, "Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry", International Orthopaedics, vol. 31, no. 6, pp. 743-751, December 2007. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:690826. [Accessed: 06 May 2024]

Metadata
Title (english)Detection of bone and cartilage-related proteins in plasma of patients with a bone fracture using liquid chromatography-mass spectrometry
AuthorLovorka Grgurević
AuthorBoris Maček
AuthorDragan Đurđević
AuthorSlobodan Vukičević
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Following bone fracture, a large number of growth factors, cytokines, and their cognate receptors involved in the repair process are active at the fracture site. To determine whether they appear in patients' blood as candidate biomarkers for following the outcome of healing, we analysed the plasma of 25 patients with an acute bone fracture following affinity plasma purification, SDS gel electrophoresis and liquid chromatography - tandem mass spectrometry (LC-MS/MS). Two hundred and thirteen nonredundant proteins were identified in the in-gel analysis of pooled plasma proteins. Gene ontology (GO) analysis indicated that a majority of detected proteins were of extracellular origin, whereas only a small number were of intracellular (cytosol and nucleus) origin. A significant proportion of detected proteins was involved in the cell growth and proliferation, transport and coagulation. Twelve proteins were potentially related to bone and cartilage metabolism, and several have not been previously identified in the plasma, including: TGF-beta induced protein IG-H(3), cartilage acidic protein 1, procollagen C proteinase enhancer protein and TGF-beta receptor III.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleInternational Orthopaedics
Numberingvol. 31, no. 6, pp. 743-751
p-ISSN0341-2695
DOIhttps://doi.org/10.1007/s00264-007-0404-z
URN:NBNurn:nbn:hr:105:690826
Publication2007-12
Document URLhttps://doi.org/10.1007/s00264-007-0404-z
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:26:06