prikaz prve stranice dokumenta Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos
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Scientific paper - Original scientific paper
Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos
Clinical and experimental immunology, 146 (2006), 1; 146-158. https://doi.org/10.1111/j.1365-2249.2006.03181.x
Grčević, Danka; Lukić, Ivan Krešimir; Kovačić, Nataša; Ivčević, Sanja; Katavić, Vedran; Marušić, Ana

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Grčević, D., Lukić, I. K., Kovačić, N., Ivčević, S., Katavić, V. & Marušić, A. (2006). Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos. Clinical and experimental immunology, 146. (1), 146-158. doi: 10.1111/j.1365-2249.2006.03181.x

Grčević, Danka, et al. "Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos." Clinical and experimental immunology, vol. 146, no. 1, 2006, pp. 146-158. https://doi.org/10.1111/j.1365-2249.2006.03181.x

Grčević, Danka, Ivan Krešimir Lukić, Nataša Kovačić, Sanja Ivčević, Vedran Katavić and Ana Marušić. "Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos." Clinical and experimental immunology 146, no. 1 (2006): 146-158. https://doi.org/10.1111/j.1365-2249.2006.03181.x

Grčević, D., et al. (2006) 'Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos', Clinical and experimental immunology, 146(1), pp. 146-158. doi: 10.1111/j.1365-2249.2006.03181.x

Grčević D, Lukić IK, Kovačić N, Ivčević S, Katavić V, Marušić A. Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos. Clinical and experimental immunology [Internet]. 2006 October [cited 2024 November 26];146(1):146-158. doi: 10.1111/j.1365-2249.2006.03181.x

D. Grčević, I. K. Lukić, N. Kovačić, S. Ivčević, V. Katavić and A. Marušić, "Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos", Clinical and experimental immunology, vol. 146, no. 1, pp. 146-158, October 2006. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:188126. [Accessed: 26 November 2024]

Metadata
Title (english)Activated T lymphocytes suppress osteoclastogenesis by diverting early monocyte/macrophage progenitor lineage commitment towards dendritic cell differentiation through down-regulation of receptor activator of nuclear factor-kappaB and c-Fos
AuthorDanka Grčević
AuthorIvan Krešimir Lukić
AuthorNataša Kovačić
AuthorSanja Ivčević
AuthorVedran Katavić
AuthorAna Marušić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Activated T lymphocytes either stimulate or inhibit osteoclastogenesis from haematopoietic progenitors in different experimental models. To address this controversy, we used several modes of T lymphocyte activation in osteoclast differentiation--mitogen-pulse, anti-CD3/CD28 stimulation and in vivo and in vitro alloactivation. Osteoclast-like cells were generated from non-adherent immature haematopoietic monocyte/macrophage progenitors in murine bone-marrow in the presence of receptor activator of nuclear factor (NF)-kappaB ligand (RANKL) and monocyte-macrophage colony-stimulating factor (M-CSF). All modes of in vivo and in vitro T lymphocyte activation and both CD4(+) and CD8(+) subpopulations produced similar inhibitory effects on osteoclastogenesis paralleled by enhanced dendritic cell (DC) differentiation. Osteoclast-inhibitory effect was associated with T lymphocyte activation and not proliferation, and could be replaced by their culture supernatants. The stage of osteoclast differentiation was crucial for the inhibitory action of activated T lymphocytes on osteoclastogenesis, because the suppressive effect was visible only on early osteoclast progenitors but not on committed osteoclasts. Inhibition was associated specifically with increased granulocyte-macrophage colony-stimulating factor (GM-CSF) expression by the mechanism of progenitor commitment toward lineages other than osteoclast because activated T lymphocytes down-regulated RANK, CD115, c-Fos and calcitonin receptor expression, and increased differentiation towards CD11c-positive DC. An activated T lymphocyte inhibitory role in osteoclastogenesis, confirmed in vitro and in vivo, mediated through GM-CSF release, may be used to counteract activated bone resorption mediated by T lymphocyte-derived cytokines in inflammatory and immune disorders. We also demonstrated the importance of alloactivation in osteoclast differentiation and the ability of cyclosporin A to abrogate T lymphocyte inhibition of osteoclastogenesis, thereby confirming the functional link between alloreaction and bone metabolism.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleClinical and experimental immunology
Numberingvol. 146, no. 1, pp. 146-158
p-ISSN0009-9104
DOIhttps://doi.org/10.1111/j.1365-2249.2006.03181.x
URN:NBNurn:nbn:hr:105:188126
Publication2006-10
Document URLhttps://doi.org/10.1111/j.1365-2249.2006.03181.x|
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:40:41