prikaz prve stranice dokumenta Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
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Scientific paper - Original scientific paper
Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
Croatian Medical Journal, 59 (2018), 5; 213-223. https://doi.org/10.3325/cmj.2018.59.213
Kafka, Anja; Karin-Kujundžić, Valentina; Šerman, Ljiljana; Bukovac, Anja; Njirić, Niko; Jakovčević, Antonia; Pećina-Šlaus, Nives

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Kafka, A., Karin Kujundžić, V., Šerman, Lj., Bukovac, A., Njirić, N., Jakovčević, A. & Pećina Šlaus, N. (2018). Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades. Croatian Medical Journal, 59. (5), 213-223. doi: 10.3325/cmj.2018.59.213

Kafka, Anja, et al. "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades." Croatian Medical Journal, vol. 59, no. 5, 2018, pp. 213-223. https://doi.org/10.3325/cmj.2018.59.213

Kafka, Anja, Valentina Karin Kujundžić, Ljiljana Šerman, Anja Bukovac, Niko Njirić, Antonia Jakovčević and Nives Pećina Šlaus. "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades." Croatian Medical Journal 59, no. 5 (2018): 213-223. https://doi.org/10.3325/cmj.2018.59.213

Kafka, A., et al. (2018) 'Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades', Croatian Medical Journal, 59(5), pp. 213-223. doi: 10.3325/cmj.2018.59.213

Kafka A, Karin Kujundžić V, Šerman Lj, Bukovac A, Njirić N, Jakovčević A, and sur.. Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades. Croatian Medical Journal [Internet]. 2018 October 31 [cited 2024 November 26];59(5):213-223. doi: 10.3325/cmj.2018.59.213

A. Kafka, et al., "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades", Croatian Medical Journal, vol. 59, no. 5, pp. 213-223, October 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:300029. [Accessed: 26 November 2024]

Metadata
Title (english)Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
AuthorAnja Kafka
AuthorValentina Karin-Kujundžić
AuthorLjiljana Šerman
AuthorAnja Bukovac
AuthorNiko Njirić
AuthorAntonia Jakovčević
AuthorNives Pećina-Šlaus
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Aim: To identify the involvement of Secreted Frizzled Related Protein 1 (SFRP1) promoter hypermethylation in different malignancy grades of astrocytoma and assess its association with beta-catenin, lymphoid-enhancer factor 1, and T-cell factor 1. Methods: Twenty-six astrocytoma samples were collected from 2008-2015. Promoter hypermethylation was evaluated by methylation-specific polymerase-chain-reaction and protein expression by immunohistochemistry and stereological analysis. The staining intensity was scored by comparing immunoreactivity with normal tissue and by using 10% and 50% cut-offs. Results: SFRP1 promoter methylation was found in 32% of astrocytomas. The number of hypermethylated samples increased in higher astrocytoma grades and was the highest in glioblastoma (P = 0.042 compared to other astrocytoma grades). There was 45.8% of samples with the lack of or weak expression of SFRP1 protein and 29.2% with strong expression. Samples with methylated promoter expressed significantly less SFRP1 than samples with unmethylated promoter (P = 0.031). Beta-catenin expression levels were elevated. Yet, glioblastomas with unmethylated SFRP1 promoter had significantly less beta-catenin (P = 0.033). Strong expression of lymphoid-enhancer factor 1 was associated to higher astrocytoma grades (P = 0.006). Conclusion: SFRP1 gene was epigenetically silenced in glioblastomas when compared to low astrocytoma grades, which may suggest that the lack of its protein is involved in astrocytoma progression.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCroatian Medical Journal
Numberingvol. 59, no. 5, pp. 213-223
p-ISSN0353-9504
DOIhttps://doi.org/10.3325/cmj.2018.59.213
URN:NBNurn:nbn:hr:105:300029
Publication2018-10-31
Document URLhttps://doi.org/10.3325/cmj.2018.59.213
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 09:47:56