prikaz prve stranice dokumenta PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants
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Scientific paper - Original scientific paper
PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants
Molecular and Cellular Probes, 22 (2008), 2; 110-114. https://doi.org/10.1016/j.mcp.2007.10.003
Mitrečić, Dinko; Mavrić, Sandra; Gajović, Srećko

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Mitrečić, D., Mavrić, S. & Gajović, S. (2008). PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants. Molecular and Cellular Probes, 22. (2), 110-114. doi: 10.1016/j.mcp.2007.10.003

Mitrečić, Dinko, et al. "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants." Molecular and Cellular Probes, vol. 22, no. 2, 2008, pp. 110-114. https://doi.org/10.1016/j.mcp.2007.10.003

Mitrečić, Dinko, Sandra Mavrić and Srećko Gajović. "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants." Molecular and Cellular Probes 22, no. 2 (2008): 110-114. https://doi.org/10.1016/j.mcp.2007.10.003

Mitrečić, D., Mavrić, S. and Gajović, S. (2008) 'PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants', Molecular and Cellular Probes, 22(2), pp. 110-114. doi: 10.1016/j.mcp.2007.10.003

Mitrečić D, Mavrić S, Gajović S. PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants. Molecular and Cellular Probes [Internet]. 2008 April [cited 2024 May 14];22(2):110-114. doi: 10.1016/j.mcp.2007.10.003

D. Mitrečić, S. Mavrić and S. Gajović, "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants", Molecular and Cellular Probes, vol. 22, no. 2, pp. 110-114, April 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:472380. [Accessed: 14 May 2024]

Metadata
Title (english)PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants
AuthorDinko Mitrečić
AuthorSandra Mavrić
AuthorSrećko Gajović
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Splotch (Pax3(sp)) and truncate (Noto(tc)) are spontaneously arisen mouse mutants with disturbed embryo development. Splotch carries a Pax3 mutation and it is characterized by the neural tube defect. Corresponding mutation in human causes Waardenburg syndrome. Truncate is Noto mutant with disturbed development of the caudal notochord. In order to establish easy genotyping procedure of these mutations, it was tested whether simple PCRs with single primer pairs could be used for this purpose. As it was necessary to differentiate sequence variants on the scale of one to several nucleotides, the approach referred to as "3' variable primer ends" was applied. The method was based on the presence of discriminating nucleotides at the 3' end of the primer sequence. This approach was successfully applied in genotyping adult mice and embryos of splotch with a 6bp deletion/insertion and truncate with a single nucleotide substitution. Described genotyping approach facilitates recognizing of these mutations and it could be in general used for detection of sequence differences in one to several nucleotides.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleMolecular and Cellular Probes
Numberingvol. 22, no. 2, pp. 110-114
p-ISSN0890-8508
DOIhttps://doi.org/10.1016/j.mcp.2007.10.003
URN:NBNurn:nbn:hr:105:472380
Publication2008-04
Document URLhttps://doi.org/10.1016/j.mcp.2007.10.003
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 10:11:38