prikaz prve stranice dokumenta The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy
  Download
PDF 158.5 KB
Scientific paper - Original scientific paper
The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy
Seizure, 17 (2008), 6; 524-530. https://doi.org/10.1016/j.seizure.2008.01.003
Bašić, Silvio; Hajnšek, Sanja; Božina, Nada; Filipčić, Igor; Sporiš, Davor; Mišlov, Damir; Posavec, Ana

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Bašić, S., Hajnšek, S., Božina, N., Filipčić, I., Sporiš, D., Mišlov, D. & Posavec, A. (2008). The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy. Seizure, 17. (6), 524-530. doi: 10.1016/j.seizure.2008.01.003

Bašić, Silvio, et al. "The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy." Seizure, vol. 17, no. 6, 2008, pp. 524-530. https://doi.org/10.1016/j.seizure.2008.01.003

Bašić, Silvio, Sanja Hajnšek, Nada Božina, Igor Filipčić, Davor Sporiš, Damir Mišlov and Ana Posavec. "The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy." Seizure 17, no. 6 (2008): 524-530. https://doi.org/10.1016/j.seizure.2008.01.003

Bašić, S., et al. (2008) 'The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy', Seizure, 17(6), pp. 524-530. doi: 10.1016/j.seizure.2008.01.003

Bašić S, Hajnšek S, Božina N, Filipčić I, Sporiš D, Mišlov D, and sur.. The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy. Seizure [Internet]. 2008 September [cited 2024 April 28];17(6):524-530. doi: 10.1016/j.seizure.2008.01.003

S. Bašić, et al., "The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy", Seizure, vol. 17, no. 6, pp. 524-530, September 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:292651. [Accessed: 28 April 2024]

Metadata
Title (english)The influence of C3435T polymorphism of ABCB1 gene on penetration of phenobarbital across the blood-brain barrier in patients with generalized epilepsy
AuthorSilvio Bašić
AuthorSanja Hajnšek
AuthorNada Božina
AuthorIgor Filipčić
AuthorDavor Sporiš
AuthorDamir Mišlov
AuthorAna Posavec
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
BACKGROUND: Epilepsy is refractory to medical treatment in about one-third of the patients. The exact pathological mechanism of epilepsy pharmacoresistance is still unclear, but a decreased antiepileptic drug (AED) uptake into the brain is suspected to play a role. P-glycoprotein (Pgp), a transmembrane transporter encoded by ABCB1 gene and located at the endothelial cells of the blood-brain barrier (BBB), has been associated with epilepsy pharmacoresistance. ----- OBJECTIVE: To analyze the effect of two ABCB1 gene polymorphisms, C3435T and G2677T/A, on phenobarbital (PB) concentrations in the cerebrospinal fluid (CSF) and serum (S) and to assess the relationship of ABCB1 polymorphisms to phenobarbital penetration across BBB in vivo and seizure frequency. ----- METHODS: CSF PB and S PB concentrations were measured in 60 patients with idiopathic primary generalized epilepsy receiving phenobarbital monotherapy. CSF/S PB concentration ratio was calculated as an index of phenobarbital penetration across BBB. The patients were genotyped for the ABCB1 gene C3435T and G2677T/A polymorphisms. Seizure frequency was recorded during the 6-month phenobarbital monotherapy. ----- RESULTS: Patients with different C3435T polymorphism had significantly different CSF PB concentrations and CSF/S PB concentration ratio. In comparison with CT heterozygotes and TT homozygotes, CC homozygotes had a significantly lower CSF PB concentration (p=0.006) and CSF/PB concentration ratio (p<0.001). G2677T/A polymorphism showed no such effect (p=0.466). CC genotype and low CSF/S PB concentration ratio correlated with increased seizure frequency. ----- CONCLUSIONS: C3435T polymorphism of ABCB1 gene was demonstrated in vivo to significantly influence the CSF/S PB concentration ratio and seizure frequency.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleSeizure
Numberingvol. 17, no. 6, pp. 524-530
p-ISSN1059-1311
DOIhttps://doi.org/10.1016/j.seizure.2008.01.003
URN:NBNurn:nbn:hr:105:292651
Publication2008-09
Document URLhttps://doi.org/10.1016/j.seizure.2008.01.003
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:08:36