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Scientific paper - Original scientific paper
Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria
Collegium Antropologicum, 33 (2009), 2; 579-585. urn:nbn:hr:105:497607
Milas, Josip; Kalenić, Smilja; Milas, Vesna; Miškulin, Maja; Vuković, Dubravka; Rudan, Stjepan; Puntarić, Dinko

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Milas, J., Kalenić, S., Milas, V., Miškulin, M., Vuković, D., Rudan, S. & Puntarić, D. (2009). Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria . Collegium Antropologicum, 33. (2), 579-585. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:497607

Milas, Josip, et al. "Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria ." Collegium Antropologicum, vol. 33, no. 2, 2009, pp. 579-585. https://urn.nsk.hr/urn:nbn:hr:105:497607

Milas, Josip, Smilja Kalenić, Vesna Milas, Maja Miškulin, Dubravka Vuković, Stjepan Rudan and Dinko Puntarić. "Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria ." Collegium Antropologicum 33, no. 2 (2009): 579-585. https://urn.nsk.hr/urn:nbn:hr:105:497607

Milas, J., et al. (2009) 'Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria ', Collegium Antropologicum, 33(2), pp. 579-585. Available at: https://urn.nsk.hr/urn:nbn:hr:105:497607 (Accessed 08 January 2025)

Milas J, Kalenić S, Milas V, Miškulin M, Vuković D, Rudan S, and sur.. Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria . Collegium Antropologicum [Internet]. 2009 June [cited 2025 January 08];33(2):579-585. Available at: https://urn.nsk.hr/urn:nbn:hr:105:497607

J. Milas, et al., "Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria ", Collegium Antropologicum, vol. 33, no. 2, pp. 579-585, June 2009. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:497607. [Accessed: 08 January 2025]

Metadata
Title (english)Effect of aminglycoside administration on the occurrence and multiplication of resistant bacteria
Title (croatian)Utjecaj uporabe aminoglikozida na nastanak i širenje otpornih bakterija
AuthorJosip Milas
AuthorSmilja Kalenić
AuthorVesna Milas
AuthorMaja Miškulin
AuthorDubravka Vuković
AuthorStjepan Rudan
AuthorDinko Puntarić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
In the prospective study the susceptibility of 41 Escherichia coli strains and 55 Pseudomonas aeruginosa strains to gentamicin, netilmicin and amikacin was tested at a 2-year interval (period I April 1998 to March 1999, and period II April to July 2001). Genotyping was performed by pulsed-field gel electrophoresis, and a clone based on 80% or 90% similarity was determined for each of the study bacteria. In 24 (32.0%) clones, strains showed no variation over 2-year interval, supporting the hypothesis on a priori susceptible strains. Transformation from susceptibility in period I to resistance in period II was demonstrated in 5 (6.7%) clones, a pattern consistent with the concept of bacterial development of resistance under the influence of antibiotics. However, there were 10 (13.3%) clones whose strains exhibited an inverse pattern. Accordingly, two-way transformation of susceptibility took place during the study period. The utilization of the study aminoglycosides had no major impact on the variation of microbial susceptibility. Changes in microbial susceptibility were found to follow some regular patterns, which were not influenced by the study aminoglycosides. Two phenomena were observed: (i) there were stable clones that did not develop resistance in spite of selective antibiotic challenge; and (ii) changes of susceptibility in isolated bacteria from both inpatient and outpatient strains of the same clone were two-way and reversible.
Abstract (croatian)
U razmaku od 2 godine (1998/1999 i 2001) ispitana je osjetljivost 41 soja Escerichie coli (E. coli), 55 sojeva Pseudomonas aeruginosa (P. aeruginosa) na gentamicin, netilmicin i amikacin. Za genotipizaciju korištena je pulsfieldgelektroforeza (PFGE). Klonovi su za svaku bakteriju određeni na temelju sličnosti od 80%, odnosno 90%. Cilj je ispitivanja promatrati djelovanje aminoglikozida na dinamiku promjena osjetljivosti sojeva nekog klona u razmaku od 2 godine u navedenim bakterijama i iz bolničkih i iz izvanbolničkih uzoraka. Najveći je broj klonova čiji su sojevi pronađeni samo u jednom ispitanom razdoblju (36, 48%) te se za njih nije mogla odrediti promjena osjetljivosti između dva razdoblja od 2 godine. U 24 klona (32%) sojevi u razmaku od 2 godine nisu mijenjali osjetljivost. Ovi podaci govore u prilog hipotezi da a priori postoje osjetljivi i otporni sojevi. U 5 (6,7%) klonova dokazana je promjena od osjetljivosti sojeva u prvom razdoblju prema otpornosti u drugom razdoblju. Ovakvo ponašanje je sukladno shvaćanju da bakterije razvijaju otpornost pod djelovanjem antibiotika. Međutim, dokazano je i postojanje 10 (13,3%) klonova čiji sojevi pokazuju suprotno: otporni sojevi iz prvog razdoblja »gube« otpornost i postaju osjetljivi u drugom razdoblju. Dakle, u promatranom su vremenu promjene osjetljivosti dvosmjerne. 88% klonova sadrže sojeve i iz bolničkih i iz izvanbolničkih uzoraka što ukazuje da nema više zatvorenih sredina. Potrošnja ispitanih aminoglikozida ne utječu na promjenu osjetljivosti.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 33, no. 2, pp. 579-585
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:497607
Publication2009-06
Document URLhttps://hrcak.srce.hr/40560
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:20:25