prikaz prve stranice dokumenta Mice genotyping using buccal swab samples: an improved method
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Scientific paper - Original scientific paper
Mice genotyping using buccal swab samples: an improved method
Biochemical Genetics, 46 (2008), 3-4; 105-112. https://doi.org/10.1007/s10528-007-9133-7
Mitrečić, Dinko; Mavrić, Sandra; Vrabec Branica, Božica; Gajović, Srećko

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Mitrečić, D., Mavrić, S., Vrabec Branica, B. & Gajović, S. (2008). Mice genotyping using buccal swab samples: an improved method. Biochemical Genetics, 46. (3-4), 105-112. doi: 10.1007/s10528-007-9133-7

Mitrečić, Dinko, et al. "Mice genotyping using buccal swab samples: an improved method." Biochemical Genetics, vol. 46, no. 3-4, 2008, pp. 105-112. https://doi.org/10.1007/s10528-007-9133-7

Mitrečić, Dinko, Sandra Mavrić, Božica Vrabec Branica and Srećko Gajović. "Mice genotyping using buccal swab samples: an improved method." Biochemical Genetics 46, no. 3-4 (2008): 105-112. https://doi.org/10.1007/s10528-007-9133-7

Mitrečić, D., et al. (2008) 'Mice genotyping using buccal swab samples: an improved method', Biochemical Genetics, 46(3-4), pp. 105-112. doi: 10.1007/s10528-007-9133-7

Mitrečić D, Mavrić S, Vrabec Branica B, Gajović S. Mice genotyping using buccal swab samples: an improved method. Biochemical Genetics [Internet]. 2008 April [cited 2024 May 14];46(3-4):105-112. doi: 10.1007/s10528-007-9133-7

D. Mitrečić, S. Mavrić, B. Vrabec Branica and S. Gajović, "Mice genotyping using buccal swab samples: an improved method", Biochemical Genetics, vol. 46, no. 3-4, pp. 105-112, April 2008. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:869960. [Accessed: 14 May 2024]

Metadata
Title (english)Mice genotyping using buccal swab samples: an improved method
AuthorDinko Mitrečić
AuthorSandra Mavrić
AuthorBožica Vrabec Branica
AuthorSrećko Gajović
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
Routine methods used to genotype mice involve isolation of DNA from partially amputated neonate's tail, toe, or ear. The inevitable drawbacks of such techniques are the animal's pain response and the increased time and funds required for DNA purification. In order to implement a noninvasive and simple protocol for mouse DNA isolation, we have improved the method based on samples collected by swabbing of the inner cheek. Combining alkaline and temperature lysis, it was possible to isolate a DNA solution ready for PCR in less than an hour. Testing the method on three different mouse lines showed that it is highly efficient, the volume of the PCR samples could be reduced to 25 microl, and fragments up to 800 bp were successfully amplified. This protocol reduces animal discomfort, shortens the time for DNA isolation, and enables amplification of larger DNA fragments with optimal success rate, thus considerably facilitating large-scale genotyping of different mouse lines.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionAccepted version
Journal titleBiochemical Genetics
Numberingvol. 46, no. 3-4, pp. 105-112
p-ISSN0006-2928
DOIhttps://doi.org/10.1007/s10528-007-9133-7
URN:NBNurn:nbn:hr:105:869960
Publication2008-04
Document URLhttp://doi.org/10.1007/s10528-007-9133-7
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:32:23