prikaz prve stranice dokumenta Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter?
  Download
PDF 165.13 KB
Scientific paper - Original scientific paper
Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter?
Collegium Antropologicum, 34 (2010), 1; 163-169. urn:nbn:hr:105:861331
Vrhovac, Radovan; Perić, Zinaida; Jurenec, Silvana; Kardum-Skelin, Ika; Jelić-Puškarić, Biljana; Jakšić, Branimir

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Vrhovac, R., Perić, Z., Jurenec, S., Kardum Skelin, I., Jelić Puškarić, B. & Jakšić, B. (2010). Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? . Collegium Antropologicum, 34. (1), 163-169. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:861331

Vrhovac, Radovan, et al. "Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? ." Collegium Antropologicum, vol. 34, no. 1, 2010, pp. 163-169. https://urn.nsk.hr/urn:nbn:hr:105:861331

Vrhovac, Radovan, Zinaida Perić, Silvana Jurenec, Ika Kardum Skelin, Biljana Jelić Puškarić and Branimir Jakšić. "Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? ." Collegium Antropologicum 34, no. 1 (2010): 163-169. https://urn.nsk.hr/urn:nbn:hr:105:861331

Vrhovac, R., et al. (2010) 'Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? ', Collegium Antropologicum, 34(1), pp. 163-169. Available at: https://urn.nsk.hr/urn:nbn:hr:105:861331 (Accessed 29 April 2024)

Vrhovac R, Perić Z, Jurenec S, Kardum Skelin I, Jelić Puškarić B, Jakšić B. Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? . Collegium Antropologicum [Internet]. 2010 March [cited 2024 April 29];34(1):163-169. Available at: https://urn.nsk.hr/urn:nbn:hr:105:861331

R. Vrhovac, Z. Perić, S. Jurenec, I. Kardum Skelin, B. Jelić Puškarić and B. Jakšić, "Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter? ", Collegium Antropologicum, vol. 34, no. 1, pp. 163-169, March 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:861331. [Accessed: 29 April 2024]

Metadata
Title (english)Post-thaw viability of cryopreserved hematopoietic progenitor cell grafts: does it matter?
Title (croatian)Vijabilnost pripravka krvotvornih matičnih stanica nakon odmrzavanja: ima li kliničko značenje ili ne?
AuthorRadovan Vrhovac
AuthorZinaida Perić
AuthorSilvana Jurenec
AuthorIka Kardum-Skelin
AuthorBiljana Jelić-Puškarić
AuthorBranimir Jakšić
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Cell viability in peripheral blood progenitor cell (PBPC) grafts and its influence on the clinical course following transplantation was evaluated in 81 consecutive transplantations (72 autologous, 9 allogeneic) performed in patients with hematological diseases. Viability of cells in PBPC grafts immediately upon collection was 98.6±3.5%, after addition of dimethyl sulfoxide (DMSO) 73.3±21.8%, and post-thaw 65.2±16.1%. It did not differ significantly between patients with different diagnoses, gender, age, type of priming used, dose of G-CSF administered or number of CD34+ cells collected. However, grafts stored for more than 60 days showed lower post-thaw viability compared to the ones thawed in the 60 days following cryopreservation (56.6±15.2% vs. 67.6±15.5%, p=0.04). Post-thaw graft viability did not influence engraftment time, but there was a predisposition towards infectious complications in the post-transplant period in patients receiving grafts with lower percentage of viable cells. They developed febrile neutropenia more often (72.2% vs. 50% of patients, p=0.05) and had more febrile days (2.4±2.6 vs. 1.5±2.3, p=0.05) following transplantation. We have demonstrated that PBPC grafts are capable of long term engraftment regardless of the graft storage time or percentage of viable cells post-thaw, which confirms the robustness of CD34+ cells during the freeze/thaw procedures carried out in daily clinical practice. Granulocyte concentration in PBPC grafts could have an influence on infectious complications following transplantation and needs to be further investigated on a larger number of patients.
Abstract (croatian)
Vijabilnost pripravka krvotvornih matičnih stanica iz periferne krvi (KMSPK) i njen utjecaj na klinički tijek nakon transplantacije istraživani su kod 81 uzastopne transplantacije (72 autologne i 9 alogeničnih) provedene u bolesnika oboljelih od hematoloških bolesti. Vijabilnost stanica KMSPK odmah nakon sakupljanja bila je 98,6±3,5%, nakon dodatka dimetil sulfoksida 73,3±21,8%, a nakon odmrzavanja 65,2±16,1%. Nije se bitno razlikovala kod bolesnika različitih dijagnoza, dobi, spola, načina na koji su KMSPK mobilizirane, dozi G-CSFa ili broju prikupljenih CD34+ stanica. Pripravci zamrznuti dulje od 60 dana imali su slabiju vijabilnost nakon odmrzavanja u usporedbi s onima koji su bili zamrznuti manje od 60 dana (56,6±15,2% prema 67,6±15,5%, p=0,04). Vijabilnost nakon odmrzavanja nije utjecala na dinamiku hematopoetskog oporavka, no kod bolesnika kojima je vijabilnost pripravka KMSPK bila slabija uočena je sklonost infektivnim komplikacijama. Češće su razvili febrilnu neutropeniju (72,2% prema 50% bolesnika, p=0,05) te su imali više febrilnih dana (2,4±2,6 prema 1,5±2,3, p=0,05) nakon transplantacije. Pokazali smo da transplantacija KMSPK dovodi do hematopoetskog oporavka neovisno o tome koliko je dugo pripravak KMSPK bio zamrznut i neovisno o vijabilnosti stanica nakon odmrzavanja. Time se potvrdila otpornost CD34+ stanica na postupke zamrzavanja i odmrzavanja koji se rutinski provode u svakodnevnoj praksi. Moguće je da koncentracija granulocita u pripravku KMSPK ima utjecaj na razvoj infektivnih komplikacija nakon transplantacije, no taj je fenomen potrebno detaljnije istražiti na većem broju bolesnika.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 34, no. 1, pp. 163-169
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:861331
Publication2010-03
Document URLhttps://hrcak.srce.hr/51258
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:41:57