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Scientific paper - Case study
Apoptosis of leukemic cells: a case report
Collegium Antropologicum, 34 (2010), 2; 705-711. urn:nbn:hr:105:010864
Vrbanus, Ljiljana; Sučić, Mirna; Marković-Glamočak, Mirjana; Ries, Sunčica; Gjadrov-Kuveždić, Koraljka; Fabijanić, Iris; Antulov, Jasenka; Petrik, József; Labar, Boris

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Vrbanus, Lj., Sučić, M., Marković Glamočak, M., Ries, S., Gjadrov Kuveždić, K., Fabijanić, I. ... Labar, B. (2010). Apoptosis of leukemic cells: a case report . Collegium Antropologicum, 34. (2), 705-711. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:010864

Vrbanus, Ljiljana, et al. "Apoptosis of leukemic cells: a case report ." Collegium Antropologicum, vol. 34, no. 2, 2010, pp. 705-711. https://urn.nsk.hr/urn:nbn:hr:105:010864

Vrbanus, Ljiljana, Mirna Sučić, Mirjana Marković Glamočak, Sunčica Ries, Koraljka Gjadrov Kuveždić, Iris Fabijanić, Jasenka Antulov, József Petrik and Boris Labar. "Apoptosis of leukemic cells: a case report ." Collegium Antropologicum 34, no. 2 (2010): 705-711. https://urn.nsk.hr/urn:nbn:hr:105:010864

Vrbanus, Lj., et al. (2010) 'Apoptosis of leukemic cells: a case report ', Collegium Antropologicum, 34(2), pp. 705-711. Available at: https://urn.nsk.hr/urn:nbn:hr:105:010864 (Accessed 08 June 2024)

Vrbanus Lj, Sučić M, Marković Glamočak M, Ries S, Gjadrov Kuveždić K, Fabijanić I, and sur.. Apoptosis of leukemic cells: a case report . Collegium Antropologicum [Internet]. 2010 June [cited 2024 June 08];34(2):705-711. Available at: https://urn.nsk.hr/urn:nbn:hr:105:010864

Lj. Vrbanus, et al., "Apoptosis of leukemic cells: a case report ", Collegium Antropologicum, vol. 34, no. 2, pp. 705-711, June 2010. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:010864. [Accessed: 08 June 2024]

Metadata
Title (english)Apoptosis of leukemic cells: a case report
Title (croatian)Apoptoza leukemijskih stanica - prikaz bolesnika
AuthorLjiljana Vrbanus
AuthorMirna Sučić
AuthorMirjana Marković-Glamočak
AuthorSunčica Ries
AuthorKoraljka Gjadrov-Kuveždić
AuthorIris Fabijanić
AuthorJasenka Antulov
AuthorJózsef Petrik
AuthorBoris Labar
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Transformation of leukemic cells is associated with delay in maturation and in apoptosis, and to altered responsiveness to growth factors. However, some studies have revealed that Fas (CD95/APO1) which mediates apoptotic signal and decrease of anti-apoptotic Bcl-2 are frequently observed in acute myeloid leukemia (AML) M4/M5 leukemic cells. The aim of the study was to compare cytomorphology and cytochemistry of bone marrow (BM) apoptotic leukemic cells to preserved peripheral blood (PB) leukemic cells in our patient, a 76-year-old man with AML-M5b treated at Zagreb University Hospital Center. BM and PB of the AL patient were analyzed after Pappenheim and cytochemical stainings, and leukemic cells were classified according to FAB and WHO classification. Analysis of PB revealed leukocytosis and 80-90% monocytic cells (46% monoblasts, 29% promonocytes and 11% monocytes). Only a few preserved monoblasts and promonocytes were found in BM, together with numerous morphologically altered cells with characteristic chromatin condensation and pyknosis of nucleus, as well as nuclear fragmentation and formation of apoptotic bodies. Thus, cytomorphology of PB leukemic cells pointed to proliferation of immature monocytic cells, and cytomorphology of BM to cell apoptosis. Cytochemistry of PB monocytic cells and BM apoptotic cells confirmed monocytic cell lineage because esterase was strongly positive in almost all BM apoptotic leukemic cells and PB leukemic cells, and esterase was completely inhibited with sodium fluoride. On the basis of these findings, AML-M5b was diagnosed in our patient. There are many possible explanations for our observation of BM leukemic cell apoptosis in a patient with AML-M5. The most reliable one is that apoptosis was induced ex vivo after BM aspiration in course of the air drying of BM specimen before staining. Mass BM leukemic cell apoptosis that was recorded in contrast to numerous preserved leukemic cells in PK could be probably connected to unfavorable ratio of relatively low concentration of cytokines in relation to high leukemic cell number in BM aspirated cytologic specimen.
Abstract (croatian)
Transformacija leukemijskih stanica povezana je s odgodom sazrijevanja, odgodom apoptoze i promijenjenim odgovorom na faktore rasta. Međutim, neke studije su pokazale da se molekula Fas (CD95/APO1), koja provodi signal apoptoze – programirane stanične smrti, te snižene vrijednosti anti-apoptotičke molekule Bcl-2, češće nalaze na leukemijskim stanicama akutnih mijeloičnih lekemija (AML) tipa M4 i M5. Cilj rada bio je usporediti citomorfologiju i citokemiju apoptotičkih leukemijskih stanica koštane srži (KS) i očuvanih leukemijskih stanica periferne krvi (PK) u našeg 76 godina starog bolesnika s M5b koji je liječen u Kliničkom bolničkom centru Zagreb. Koštana srž i periferna krv bolesnika analizirane su nakon standardnog bojenja po Pappenheimu i citokemijskih bojenja i akutna leukemija (AL) je klasificirana prema kriterijima FAB (French-American-British) i SZO (Svjetska zdravstvena organizacija). Analiza PK je pokazala leukocitozu i 80–90% monocitnih stanica (46% monoblasta, 29% promonocita i 11% monocita). U KS su nađene brojne morfološki promijenjene stanice s karakterističnom kondenzacijom kromatina i piknotičkim jezgrama, fragmentacijom jezgri, apoptotička tjelešca, te tek nekoliko očuvanih monoblasta i promonocita. Citomorfologija leukemijskih stanica PK upućivala je na proliferaciju monocitnih stanica, dok je citomorfologija KS upućivala na apoptozu stanica. Citokemija monocitnih stanica PK i apoptotičkih stanica KS potvrdila je monocitnu liniju stanica jer je u gotovo svim leukemijskim apoptotičnim stanicama KS i leukemijskim stanicama PK nespecifična esteraza bila izrazito pozitivna i njena je aktivnost potpuno inhibirana natrijevim fluoridom. Na temelju morfologije i citokemije leukemijskih stanica postavljena je dijagnoza AL-M5b. Brojni poremećaji u leukemijskim stanicama teoretski bi mogli potaknuti apoptozu u stanicama KS u našeg bolesnika s M5b. Do apoptoze je, međutim, najvjerojatnije došlo ex vivo nakon što je učinjena dijagnostička punkcija koštane srži, dok su se citološki uzorci sušili na zraku tijekom 2 sata prije postupka bojenja. Objašnjenje za tako velik broj apoptotičkih leukemijskih stanica u koštanoj srži nasuprot brojnim očuvanim leukemijskim stanicama u PK moglo bi biti povezano s nepovoljnim odnosom relativno niske koncentracije citokina u odnosu na izrazito velik broj leukemijskih stanica u aspiratu koštane srži.
Keywords (english)
Languageenglish
Publication typeScientific paper - Case study
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 34, no. 2, pp. 705-711
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:010864
Publication2010-06
Document URLhttps://hrcak.srce.hr/56541
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 11:46:54