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Scientific paper - Original scientific paper
AXIN-1 protein expression and localization in glioblastoma
Collegium Antropologicum, 35 (2011), S. 1; 101-106. urn:nbn:hr:105:938585
Pećina-Šlaus, Nives; Nikuševa Martić, Tamara; Kokotović, Tomislav; Kušec, Vesna; Tomas, Davor; Hrašćan, Reno

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Pećina Šlaus, N., Nikuševa Martić, T., Kokotović, T., Kušec, V., Tomas, D. & Hrašćan, R. (2011). AXIN-1 protein expression and localization in glioblastoma . Collegium Antropologicum, 35. (S. 1), 101-106. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:938585

Pećina Šlaus, Nives, et al. "AXIN-1 protein expression and localization in glioblastoma ." Collegium Antropologicum, vol. 35, no. S. 1, 2011, pp. 101-106. https://urn.nsk.hr/urn:nbn:hr:105:938585

Pećina Šlaus, Nives, Tamara Nikuševa Martić, Tomislav Kokotović, Vesna Kušec, Davor Tomas and Reno Hrašćan. "AXIN-1 protein expression and localization in glioblastoma ." Collegium Antropologicum 35, no. S. 1 (2011): 101-106. https://urn.nsk.hr/urn:nbn:hr:105:938585

Pećina Šlaus, N., et al. (2011) 'AXIN-1 protein expression and localization in glioblastoma ', Collegium Antropologicum, 35(S. 1), pp. 101-106. Available at: https://urn.nsk.hr/urn:nbn:hr:105:938585 (Accessed 22 November 2024)

Pećina Šlaus N, Nikuševa Martić T, Kokotović T, Kušec V, Tomas D, Hrašćan R. AXIN-1 protein expression and localization in glioblastoma . Collegium Antropologicum [Internet]. 2011 January [cited 2024 November 22];35(S. 1):101-106. Available at: https://urn.nsk.hr/urn:nbn:hr:105:938585

N. Pećina Šlaus, T. Nikuševa Martić, T. Kokotović, V. Kušec, D. Tomas and R. Hrašćan, "AXIN-1 protein expression and localization in glioblastoma ", Collegium Antropologicum, vol. 35, no. S. 1, pp. 101-106, January 2011. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:938585. [Accessed: 22 November 2024]

Metadata
Title (english)AXIN-1 protein expression and localization in glioblastoma
Title (croatian)Izraženost i smještaj proteina aksina u glioblastomima
AuthorNives Pećina-Šlaus
AuthorTamara Nikuševa Martić
AuthorTomislav Kokotović
AuthorVesna Kušec
AuthorDavor Tomas
AuthorReno Hrašćan
Author's institutionUniversity of Zagreb
School of Medicine
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract (english)
The etiology and pathogenesis of tumors of the central nervous system are still inadequately explained. In the present study the expression patterns of a critical molecular component of wnt signaling pathway – axin 1 was investigated in 42 patients with glioblastoma, the most aggressive form of glial tumors. Immunostaining and image analysis revealed the quantity and localization of the protein. Downregulation of this tumor suppressor expression was observed in 31% of tumors when compared to the levels of axin in healthy brain tissues. Axin was observed in the cytoplasm in 69% of glioblastoma samples, in 21.4% in both the cytoplasm and nucleus and 9.5% had expression solely in the nucleus. Mean values of relative axin’s expression obtained by image analysis showed that the highest relative quantity of axin was measured when the protein was in the nucleus and the lowest relative quantity of axin when the protein was localized in the cytoplasm. Investigation on axin’s existence at the subcellular level in glioblastomas suggests that axin’s expression and spatial regulation is a dynamic process. Despite increasing knowledge on glioma biology and genetics, the prognostic tools for glioblastoma still need improvement. Our findings on expression of axin 1 may contribute to better understanding of glioblastoma molecular profile.
Abstract (croatian)
Etiologija i patogeneza tumora središnjeg živčanog sustava još uvijek je nedovoljno razjašnjena. U ovoj studiji istraživani su obrasci ekspresije proteina aksina, izuzetno važne molekularne komponente signalnog puta Wnt, u 42 uzorka glioblastoma čovjeka. Imunohistokemijskom metodom i image analizom utvrđena je jačina ekspresije i lokalizacija proteina u stanicama. Smanjena ekspresija ovog tumor supresorskog proteina uočena je u 31% analiziranih tumora usporedbom s razinom ekspresije aksina u normalnom tkivu mozga. U 69% glioblastoma aksin je pronađen u citoplazmi, u 21,4% u jezgri i citoplazmi, dok je u 9,5% glioblastoma lokalizacija bila isključivo u jezgrama. Srednje vrijednosti relativne ekspresije proteina aksina dobivene image analizom pokazuju da je najveća relativna količina aksina izmjerena kada je protein lokaliziran u jezgri, a najmanja relativna količina aksina kada je lokaliziran u citoplazmi. Rezultati istraživanja aksina u glioblastomima na unutarstaničnoj razini pokazuju da je izraženost aksina i njegova prostorna regulacija vrlo dinamičan proces. Unatoč produbljivanju spoznaja o biologiji i genetici glijalnih tumora, saznanja o prognostičkim parametrima za glioblastom još uvijek nisu dostatna. Naši rezultati o razini izraženosti proteina aksina zasigurno će doprinijeti boljem razumijevanju molekularnog profila glioblastoma, te ponuditi potencijalne prognostičke značajke.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleCollegium Antropologicum
Numberingvol. 35, no. S. 1, pp. 101-106
p-ISSN0350-6134
URN:NBNurn:nbn:hr:105:938585
Publication2011-01
Document URLhttps://hrcak.srce.hr/64050
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-03-28 12:02:01