prikaz prve stranice dokumenta Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju
Embargo period 2026-04-16
doctoral thesis
Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju
Zagreb: University of Zagreb, School of Medicine, 2024. urn:nbn:hr:105:136902
Franić, Dina
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Franić, D. (2024). Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:136902

Franić, Dina. "Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju." Doctoral thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:136902

Franić, Dina. "Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju." Doctoral thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:136902

Franić, D. (2024). 'Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju', Doctoral thesis, University of Zagreb, School of Medicine, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:105:136902

Franić D. Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2024 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:136902

D. Franić, "Selektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:105:136902

Metadata
TitleSelektivna razgradnja pogrešno smotanih proteina u stanicama kvasca Saccharomyces cerevisiae u mirovanju
Title (english)Selective degradation of misfolded proteins in quiescent yeast Saccharomyces cerevisiae
AuthorDina Franić
MentorMirta Boban (mentor)
Committee memberDora Višnjić (predsjednik povjerenstva)
Committee memberSvjetlana Kalanj-Bognar (član povjerenstva)
Committee memberMelita Šalković-Petrišić (član povjerenstva)
Committee memberKristina Mlinac Jerković (član povjerenstva)
Committee memberŽeljka Krsnik (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2024-04-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Kako bi se zaštitile od nakupljanja pogrešno smotanih proteina, stanice su razvile mehanizme za kontrolu kvalitete proteina, uključujući put selektivne razgradnje sustavom ubikvitin-proteasoma. Prethodna istraživanja kontrole kvalitete proteina uglavnom su provedena u stanicama koje se aktivno dijele, međutim mnoge stanice, poput neurona i matičnih stanica u mirovanju, se ne dijele. U ovoj disertaciji istražili smo puteve selektivne razgradnje proteina u stanicama u mirovanju, koristeći modelni organizam, kvasac Saccharomyces cerevisiae. Po ulasku stanica kvasca u mirovanje aktivira se autofagija, a veliki dio proteasoma reorganizira se u granule u kojima proteasomi vjerojatno nisu aktivni, stoga nije bilo jasno oslanjaju li se stanice u mirovanju na sustav ubikvitin-proteasoma za eliminaciju pogrešno smotanih proteina. U ovom radu uspostavili smo ekspresiju modelnih pogrešno smotanih proteina tGnd1, stGnd1 i Ubc9ts u stanicama u mirovanju, koristeći PIR3- i Z-promotore. Pokazali smo da stanice u mirovanju prepoznaju pogrešno smotane proteine te ih usmjeravaju u selektivnu razgradnju, čak i u kasnijim fazama mirovanja. Nadalje, razgradnja je ovisila o aktivnosti E3 ligaza ubikvitina Ubr1 i San1, te proteasomu, što upućuje na sličan put razgradnje kao u proliferirajućim stanicama. Sveukupno rezultati pokazuju da stanice kvasca u mirovanju zadržavaju funkcionalnu kontrolu kvalitete proteina, te da se ona primarno temelji na selektivnoj razgradnji proteina.
Abstract (english)
To prevent the negative impact of misfolded protein accumulation, cells have developed protein quality control pathways, including selective degradation by the ubiquitin-proteasome system. Previous research on protein quality control has mostly been conducted in actively dividing cells. However, many cells, such as neurons and quiescent stem cells, are non-dividing. In this thesis, we investigated selective protein degradation in quiescent cells, using yeast Saccharomyces cerevisiae as a model organism. Upon entry into quiescence, yeast cells induce autophagy, and a large portion of the proteasomes relocalizes into cytoplasmic granules, presumably in an inactive form. Therefore, it has been unclear whether quiescent cells rely on the ubiquitin-proteasome system for the elimination of misfolded proteins. In this study, we established an expression system of model misfolded proteins tGnd1, stGnd1, and Ubc9ts in quiescent cells using PIR3- and Z-promoters. We show that quiescent cells recognize misfolded proteins and direct them to selective degradation, even in the later stages of quiescence. Furthermore, degradation was dependent on the E3-ubiquitin ligases Ubr1 and San1 and the proteasome, suggesting a similar pathway as in proliferating cells. Overall, the results show that quiescent yeast cells maintain functional protein quality control, which is primarily based on selective protein degradation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:136902
ProjectNumber: PZS-2019-02-3610
Title: Kontrola kvalitete proteina putem selektivne razgradnje u stanicama u mirovanju
Title: Degradation-mediated protein quality control in quiescent cells
Acronym: Q-CTRL
Leader: Mirta Boban
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: Nema
ProjectNumber: KK.01.1.1.01.0007
Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi
Leader: Miloš Judaš
Jurisdiction: eu
Funder: EK
Study programmeTitle: Neuroscience
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent107 str.
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2026-04-16
Terms of useLicence In copyright icon
Created on2024-04-19 09:07:50