prikaz prve stranice dokumenta Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice
Embargo period 2025-03-28
doctoral thesis
Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice
Zagreb: University of Zagreb, School of Medicine, 2024. urn:nbn:hr:105:824633
Justić, Helena
University of Zagreb
School of Medicine

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Justić, H. (2024). Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice (Doctoral thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:824633

Justić, Helena. "Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice." Doctoral thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:824633

Justić, Helena. "Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice." Doctoral thesis, University of Zagreb, School of Medicine, 2024. https://urn.nsk.hr/urn:nbn:hr:105:824633

Justić, H. (2024). 'Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice', Doctoral thesis, University of Zagreb, School of Medicine, accessed 22 May 2024, https://urn.nsk.hr/urn:nbn:hr:105:824633

Justić H. Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice [Doctoral thesis]. Zagreb: University of Zagreb, School of Medicine; 2024 [cited 2024 May 22] Available at: https://urn.nsk.hr/urn:nbn:hr:105:824633

H. Justić, "Uloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice", Doctoral thesis, University of Zagreb, School of Medicine, Zagreb, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:105:824633

Metadata
TitleUloga bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice
Title (english)The role of bradykinin receptor type 2 in the development of brain and retinal ischemic lesion
AuthorHelena Justić
MentorMarina Radmilović (mentor)
Committee memberNives Pećina-Šlaus (predsjednik povjerenstva)
Committee memberTomislav Jukić (član povjerenstva)
Committee memberAleksandra Dugandžić (član povjerenstva)
Committee memberHrvoje Banfić (član povjerenstva)
Committee memberNataša Jovanov Milošević (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
Zagreb
Defense date and country2024-03-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Usprkos velikom broju kliničkih i pretkliničkih dokaza o korelaciji ishemije mozga i mrežnice, malo je pažnje posvećeno zajedničkim patološkim mehanizmima razvoja ishemijske ozljede istih. Cilj ovog istraživanja bio je longitudinalnim in vivo praćenjem razjasniti ulogu bradikininskog receptora tipa 2 u razvoju ishemijske ozljede mozga i mrežnice te odrediti primjenjivost in vivo snimanja mrežnične vaskulature za određivanje cerebrovaskularnih promjena u mišjem modelu moždanog udara. Usporedbom Koizumi i Longa zahvata okluzije srednje moždane arterije i dugotrajnim praćenjem statusa cerebralne vaskularne perfuzije magnetskom rezonancijom pokazano je da kod Koizumi zahvata ishemiju prati kronična hipoperfuzija i ishemija mozga i mrežnice, za razliku od Longa zahvata, u kojem nakon ishemije nastupa potpuna reperfuzija koja rezultira izraženijom cerebralnom atrofijom bez znakova oštećenja mrežnice. Usporedbom miševa s onemogućenim genom za bradikininski receptor tipa 2 te njihovih kontrola nakon Koizumi zahvata, dugotrajnim praćenjem razvoja ishemije magnetskom rezonancijom, fotografijom i fluoresceinskom angiografijom očne pozadine, testovima ponašanja i mjerenjem vaskularne propusnosti pokazano je da nedostatak bradikininskog receptora tipa 2 u akutnoj fazi ishemije smanjuje oštećenje i propusnost cerebralne vaskulature, a kronično povećava atrofiju moždanog tkiva i funkcionalni deficit. U mrežnici je pokazano slično djelovanje, odnosno povećanje vaskularne propusnosti u subakutnoj fazi, te povećanje ishemijskog oštećenja u kroničnoj fazi ishemije.
Abstract (english)
Despite ample clinical and preclinical evidence correlating cerebral and retinal ischemia, not enough attention has been paid to common pathological mechanisms of ischemic lesion development in these tissues. This research aimed to clarify the role of bradykinin receptor type 2 in the development of brain and retinal ischemia and to examine the applicability of in vivo retinal vascular imaging to determine cerebrovascular changes in a murine model of stroke. Comparing the Koizumi and Longa middle cerebral artery occlusion procedures by long-term cerebrovascular perfusion monitoring with magnetic resonance imaging showed that ischemia was followed by chronic hypoperfusion in both brain and retina in the Koizumi model. In contrast, complete reperfusion occurred in the Longa model, resulting in greater cerebral atrophy without retinal damage. By comparing bradykinin receptor type 2 knock-out mice and their controls after the Koizumi procedure using long-term monitoring with magnetic resonance, fundus photography, fluorescein angiography, behavioral tests and vascular permeability measurements, bradykinin receptor type 2 deficiency has been shown to reduce acute cerebral vascular permeability, while increasing cerebral atrophy and functional deficit in the chronic phase. Retinal findings showed a similar effect, increased subacute vascular permeability and increased ischemic damage in the chronic phase of ischemia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:824633
ProjectNumber: UIP-2017-05-8082
Title: Uloga bradikinina u ishemiji mozga i mrežnice u mišjim modelima dijabetesa
Title: The role of bradykinin in brain and retinal ischemia in diabetic murine models
Acronym: BRADISCHEMIA
Leader: Marina Radmilović
Jurisdiction: Croatia
Funder: Hrvatska zaklada za znanost
Funding stream: Installation Research Projects
Study programmeTitle: Neuroscience
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent155 str.
File originBorn digital
Access conditionsEmbargoed access
Embargo expiration date: 2025-03-28
Terms of useLicence In copyright icon
Created on2024-04-26 07:18:41