prikaz prve stranice dokumenta Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
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Scientific paper - Original scientific paper
Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
International Journal of Molecular Sciences, 24 (2023), 17; 13574. https://doi.org/10.3390/ijms241713574
Skrzydlewska, Elżbieta; Łuczaj, Wojciech; Biernacki, Michał; Wójcik, Piotr; Jarocka-Karpowicz, Iwona; Orehovec, Biserka; Baršić, Bruno; Tarle, Marko; Kmet, Marta; Lukšić, Ivica; Marušić, Zlatko; Bauer, Georg; Žarković, Neven

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

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Skrzydlewska, E., Łuczaj, W., Biernacki, M., Wójcik, P., Jarocka Karpowicz, I., Orehovec, B. ... Žarković, N. (2023). Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients. International Journal of Molecular Sciences, 24. (17). doi: 10.3390/ijms241713574

Skrzydlewska, Elżbieta, et al. "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients." International Journal of Molecular Sciences, vol. 24, no. 17, 2023. https://doi.org/10.3390/ijms241713574

Skrzydlewska, Elżbieta, Wojciech Łuczaj, Michał Biernacki, Piotr Wójcik, Iwona Jarocka Karpowicz, Biserka Orehovec, Bruno Baršić, et al. "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients." International Journal of Molecular Sciences 24, no. 17 (2023). https://doi.org/10.3390/ijms241713574

Skrzydlewska, E., et al. (2023) 'Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients', International Journal of Molecular Sciences, 24(17). doi: 10.3390/ijms241713574

Skrzydlewska E, Łuczaj W, Biernacki M, Wójcik P, Jarocka Karpowicz I, Orehovec B, and sur.. Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients. International Journal of Molecular Sciences [Internet]. 2023 September 01 [cited 2024 November 05];24(17). doi: 10.3390/ijms241713574

E. Skrzydlewska, et al., "Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients", International Journal of Molecular Sciences, vol. 24, no. 17, September 2023. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:105:969039. [Accessed: 05 November 2024]

Metadata
Title (english)Preliminary Comparison of Molecular Antioxidant and Inflammatory Mechanisms Determined in the Peripheral Blood Granulocytes of COVID-19 Patients
AuthorElżbieta Skrzydlewska
AuthorWojciech Łuczaj
AuthorMichał Biernacki
AuthorPiotr Wójcik
AuthorIwona Jarocka-Karpowicz
AuthorBiserka Orehovec
AuthorBruno Baršić
AuthorMarko Tarle
AuthorMarta Kmet
AuthorIvica Lukšić
AuthorZlatko Marušić
AuthorGeorg Bauer
AuthorNeven Žarković
Author's institutionUniversity of Zagreb
School of Medicine
(Department of Maxillofacial Surgery and Dentistry)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Surgery
Abstract (english)
The aim of this study was to evaluate selected parameters of redox signaling and inflammation in the granulocytes of COVID-19 patients who recovered and those who died. Upon admission, the patients did not differ in terms of any relevant clinical parameter apart from the percentage of granulocytes, which was 6% higher on average in those patients who died. Granulocytes were isolated from the blood of 15 healthy people and survivors and 15 patients who died within a week, and who were selected post hoc for analysis according to their matching gender and age. They differed only in the lethal outcome, which could not be predicted upon arrival at the hospital. The proteins level (respective ELISA), antioxidant activity (spectrophotometry), and lipid mediators (UPUPLC–MS) were measured in the peripheral blood granulocytes obtained via gradient centrifugation. The levels of Nrf2, HO-1, NFκB, and IL-6 were higher in the granulocytes of COVID-19 patients who died within a week, while the activity of cytoplasmic Cu,Zn-SOD and mitochondrial Mn-SOD and IL-2/IL-10 were lower in comparison to the levels observed in survivors. Furthermore, in the granulocytes of those patients who died, an increase in pro-inflammatory eicosanoids (PGE2 and TXB2), together with elevated cannabinoid receptors 1 and 2 (associated with a decrease in the anti-inflammatory 15d-PGJ2), were found. Hence, this study suggests that by triggering transcription factors, granulocytes activate inflammatory and redox signaling, leading to the production of pro-inflammatory eicosanoids while reducing cellular antioxidant capacity through SOD, thus expressing an altered response to COVID-19, which may result in the onset of systemic oxidative stress, ARDS, and the death of the patient.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleInternational Journal of Molecular Sciences
Numberingvol. 24, no. 17, 13574
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms241713574
URN:NBNurn:nbn:hr:105:969039
Publication2023-09-01
Document URLhttps://www.mdpi.com/1422-0067/24/17/13574
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-05-22 11:38:31