Perineuronal nets (PNN) are structures formed when cells secrete molecules of chondroitin sulphate proteoglycan, which condense and surround certain neurons and their axons. Today it is known that they perform a wide variety of functions in the normal development and diseased brain. In this research, PNN of the human fetal and children brain were visualized using a histochemical staining with Wisteria floribunda agglutinin which is a well-established marker of the perineuronal nets and the extracellular matrix rich in. The aim of the study was to determine the intensity of expression, as well as its spatio-temporal allocation in the human telencephalon during development, focusing primarily on the subplate zone but also on the cortical plate. The results show that the perineuronal nets do have a specific pattern of expression, as well as a spatio-temporal distribution in the subplate zone. In the 13th week of gestation, perineuronal nets are expressed in the pre-subplate zone as a perisomatic staining in the dorzo-lateral telencephalic wall. After that, until the 25th week of gestation, they are expressed as a diffuse staining in the subplate zone, mostly around the prospective insula. Until the 33rd gestation week the perineuroneal nets are very faintly seen in the subplate zone. After that period they are expressed as an intense staining in the remnants of subplate zone. These results, as well as many others, suggest an important role that the perineuronal nets have during development and normal functioning of the brain.