prikaz prve stranice dokumenta Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu
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master's thesis
Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu
Zagreb: University of Zagreb, School of Medicine, 2014. urn:nbn:hr:105:230346
Skelin, Marta
University of Zagreb
School of Medicine
Department of Histology and Embryology

Institutional repository: Dr Med - University of Zagreb School of Medicine Digital Repository

Cite this document

Skelin, M. (2014). Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu (Master's thesis). Zagreb: University of Zagreb, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:105:230346

Skelin, Marta. "Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu." Master's thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:230346

Skelin, Marta. "Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu." Master's thesis, University of Zagreb, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:105:230346

Skelin, M. (2014). 'Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu', Master's thesis, University of Zagreb, School of Medicine, accessed 23 December 2024, https://urn.nsk.hr/urn:nbn:hr:105:230346

Skelin M. Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu [Master's thesis]. Zagreb: University of Zagreb, School of Medicine; 2014 [cited 2024 December 23] Available at: https://urn.nsk.hr/urn:nbn:hr:105:230346

M. Skelin, "Epitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu", Master's thesis, University of Zagreb, School of Medicine, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:105:230346

Metadata
TitleEpitel cerviksa miša tijekom spolnog ciklusa i ekspresija GABA receptora u cervikalnom epitelu
Title (english)Cervical epithelium of the mouse during estrous cycle and the expression of GABA receptor in the cervical epithelium
AuthorMarta Skelin
MentorMarija Ćurlin (mentor)
Committee memberSrećko Gajović (predsjednik povjerenstva)
Committee memberDinko Mitrečić (član povjerenstva)
Committee memberMarija Ćurlin (član povjerenstva)
GranterUniversity of Zagreb
School of Medicine
(Department of Histology and Embryology)
Zagreb
Defense date and country2014-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Cytology, Histology and Embryology
Abstract
Kao početni korak koji bi omogućio daljnje istraživanje uloge cervikalne sluzi u plodnosti, ovaj rad istražuje može li miš poslužiti kao eksperimentalni model za proučavanje karakteristika i regulacije lučenja cervikalne sluzi. U sklopu ostvarivanja prvog cilja, proučavanja promjena građe epitela sluznice cerviksa i lučenja sluzi tijekom spolnog ciklusa, provjerava se postupak uzimanja sekreta iz rodnice kao metode određivanja faze spolnog ciklusa u kojoj se miš nalazi. Nadalje, citološka analiza sekreta iz rodnice povezuje se s histološki prikazanim promjenama cervikalnog epitela i prisutnošću sluzi u pojedinim fazama. Histološko-kemijskim metodama dokazuje se da se proizvodnja sluzi odvija u cilindričnom epitelu cervikalne sluznice. Postizanje drugog cilja, istraživanje prisutnosti receptora neurotransmitera GABA u žljezdanim stanicama mišjeg cerviksa, uključuje dokazivanje ekspresije estrogenskog receptora RTPCR-om, kao i receptora za neurotransmiter GABA. Pod pretpostavkom da GABA receptor igra ulogu u regulaciji ciklusa, imunohistokemijom je prikazana njegova raspodjela u tkivu cerviksa i na taj način opravdava nastavak istraživanja usmjerenog na ovaj mehanizam regulacije lučenja cervikalne sluzi.
Abstract (english)
As a starting point to enable further exploration of the role of cervical mucus in fertility, this paper investigates whether a mouse can be used as an experimental model in studying characteristics and regulation of cervical mucus secretion. To achieve our first goal, the study of changes in the epithelial structure of the cervical mucosa and mucus secretion during the estrous cycle, the procedure of collecting samples of vaginal secretion to determine in which phase of the estrous cycle the mouse is, is reviewed. Furthermore, cytological analysis of the vaginal secretion is associated with the histologically shown changes in the cervical epithelium and mucus presence. Histologically-chemical methods prove that mucus secretion takes place in columnar epithelium of the cervical mucosa. The process of fulfilling the second objective, the research of neurotransmitter GABA receptor presence in the glandular epithelium of the mouse cervix, includes documenting estrogen receptor presence by RTPCR, as well as receptors for the neurotransmitter GABA. Assuming that the GABA receptor plays a role in the cycle regulation, immunohistochemistry shows its distribution in the cervical tissue and thus justifies further research on this mechanism of secretion regulation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:105:230346
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-11-29 10:26:43