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undergraduate thesis
Genotoksikološki utjecaj kemikalija na kromosomske aberacije
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2015. urn:nbn:hr:152:674621
Vrbančić, Kristina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Vrbančić, K. (2015). Genotoksikološki utjecaj kemikalija na kromosomske aberacije (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:674621

Vrbančić, Kristina. "Genotoksikološki utjecaj kemikalija na kromosomske aberacije." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2015. https://urn.nsk.hr/urn:nbn:hr:152:674621

Vrbančić, Kristina. "Genotoksikološki utjecaj kemikalija na kromosomske aberacije." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2015. https://urn.nsk.hr/urn:nbn:hr:152:674621

Vrbančić, K. (2015). 'Genotoksikološki utjecaj kemikalija na kromosomske aberacije', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:674621

Vrbančić K. Genotoksikološki utjecaj kemikalija na kromosomske aberacije [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2015 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:152:674621

K. Vrbančić, "Genotoksikološki utjecaj kemikalija na kromosomske aberacije", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:152:674621

Metadata
TitleGenotoksikološki utjecaj kemikalija na kromosomske aberacije
Title (english)Genotoxical influence of chemicals on chromosome abberations
AuthorKristina Vrbančić
MentorJasenka Wagner (mentor)
Committee memberMarija Heffer (član povjerenstva)
Committee memberMartina Smolić (član povjerenstva)
Committee memberJasenka Wagner (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2015-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Test na kromosomske aberacije nam pomaže identificirati strukturalna kromosomska oštećenja na uzgojenim stanicama. Identifikacijom kromosomskih oštećenja može se procijeniti štetnost kemikalije ili zračenja na ljudski organizam. Cilj istraživanja bio je ispitati genotoksikološki i citotoksični učinak metanola, ksilola i octene kiseline na nastanak in vitro kromosomskih aberacija u limfocitima periferne krvi. Ispitanici su osobe mlađe od 40 godina koje ne rade s kemikalijama niti u zoni zračenja. Svaki ispitanik je sam sebi služio kao kontrola. Metoda testa na kromosomske aberacije zasniva se na 45-satnom uzgoju limfocita iz periferne krvi u hranjivom mediju u prisutnosti fitohemaglutinina i L-glutamina i analizi metafaza svake koncentracije kemikalije kod svakog ispitanika. Testirane su tri koncentracije od svake kemikalije i kontrola. Iz rezultata je vidljivo da broj kromosomskih aberacija raste s najvećom koncentracijom pojedine kemikalije. Statistički značajnu razliku smo dobili između koncentracija ksilola i octene kiseline (P < 0,05). Ovim istraživanjem možemo potvrditi hipotezu jer smo pokazali da kemikalije utječu na pojavu kromosomskih aberacija i na smanjenje mitotičkog indeksa
Abstract (english)
The chromosome aberration test method is used to identify structural damage to the chromosome or to other cultivated cells. By identifying the damage done to a chromosome one can assess the damage potential of a certain chemical substance or radiation. The purpose of this research was to test the genotoxicological and cytotoxic effect of methanol, xylol and acetic acid on the creation of “in vitro“ chromosome aberrations in lymphocytes of peripheral blood. Test subjects were people aged up to 40 and who do not handle chemicals nor are in contact with radiation. Each test subject served as his own test reference. This test method is based on a 45 hour lymphocyte cultivation in a nutritious medium in the presence of phytohaemagglutinin and L-glutamine and a metaphase analysis of every chemical concentration in every test subject. Three different chemical concentrations of each chemical were tested and a control test was done. Given the results one can conclude that the number of chromosome aberrations increases when the concentration of a certain chemical rises. Statistically, a substantial difference has been observed at different xylol and acetic acid concentration (P<0,05). This research proves that the hypothesis is correct because it has shown that certain chemicals influence the creation of chromosome aberrations, as well as the decrease of the mitotic index.  
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:674621
Study programmeTitle: University undergraduate study; specializations in: medical laboratory diagnostics
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-05-05 11:29:12