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undergraduate thesis
Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2015. urn:nbn:hr:152:442540
Galić, Mia
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Galić, M. (2015). Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:442540

Galić, Mia. "Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2015. https://urn.nsk.hr/urn:nbn:hr:152:442540

Galić, Mia. "Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2015. https://urn.nsk.hr/urn:nbn:hr:152:442540

Galić, M. (2015). 'Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:442540

Galić M. Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2015 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:152:442540

M. Galić, "Ispitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:152:442540

Metadata
TitleIspitivanje genetičke nestabilnosti primjenom in vitro analize izmjene sestrinskih kromatida
Title (english)Investigation of genetic instability using in vitro analysis of sister chromatide exchange
AuthorMia Galić
MentorJasenka Wagner (mentor)
Committee memberMarija Heffer (predsjednik povjerenstva)
Committee memberMartina Smolić (član povjerenstva)
Committee memberJasenka Wagner (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2015-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Cilj istraživanja je bio utvrditi uzrokuje li izlaganje ksilolu, metanolu i octenoj kiselini veći rizik za nastanak oštećenja DNA. Ispitanici su osobe mlađe od 40 godina koje ne rade s kemikalijama niti u zoni zračenja. Analiza SCE zasniva se na 72-satnome uzgoju limfocita iz periferne krvi u hranjivom mediju u prisutnosti 5-bromodeoksiuridina (BrdU) i fitohemaglutinina (PHA). Za svaku kemikaliju testirane su tri različite koncentracije te kontrola. Rezultati su pokazali da kemikalije uzrokuju povećanje broja izmjena sestrinskih kromatida u odnosu na kontrolu. Pri svim koncentracijama kemikalija postoji statistički značajno smanjenje mitotičkog indeksa (P<0,05). Ovim istraživanjem je pokazano kako sve testirane kemikalije uzrokuju povećan broj rekombinacijskih popravaka u limfocitima periferne krvi te imaju antiproliferativni učinak povezan s genotoksičnošću. Biomonitoring je važan dio zdravstvenog nadzora osoba profesionalno izloženih štetnim utjecajima. Zasniva se na mjerenju bioloških pokazatelja koji ukazuju na rane biološke učinke koji prethode pojavi zloćudnih i drugih bolesti. Rutinsko provođenje analize SCE može indirektno pokazati koliko su dobre mjere zaštite na radnom mjestu. KLJUČNE RIJEČI: genetička nestabilnost; izmjene sestrinskih kromatida (SCE); ksilol; metanol; mitotički indeks
Abstract (english)
Sister chromatid exchange from in vitro analysis (SCE) is a cytogenetic method used to control professionally exposed population by means of mutagenic or carcinogenic chemicals. It is the indirect indicator of the level of damage present in the DNA before duplication. The purpose was to determine if the exposure of xylene, methanol and acetic acid poses increased risk of DNA damage. The participants were people younger than 40 who do not work with chemicals or in a radiation zone. The analysis of SCE is based on the 72-hour cultivation of peripheral blood lymphocytes in the nutrient medium in the presence of 5-bromodeoxyuridine (BrdU) and phytohaemagglutinin (PHA). For each chemical, three different concentrations and control are tested. The results demonstrated that the chemicals cause an increase in the number of sister chromatid exchange in relation to the controls. There is a statistically significant reduction of mitotic index (P <0.05) at all concentrations of tested chemicals. This research demonstrates that all of tested chemicals cause an increased number of recombination repair in peripheral lymphocytes and have an antiproliferative effect associated with genotoxicity. Biomonitoring is an important part of health checkups of people occupationally exposed to health hazards. It is based on the measurement of biological indicators which indicate early biological effects that precede the onset of malignancies and other diseases. A routine analysis of SCE may indirectly show the quality of safety measures at a workplace.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:442540
Study programmeTitle: University undergraduate study; specializations in: medical laboratory diagnostics
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2016-06-24 11:59:17