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master's thesis
Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. urn:nbn:hr:152:838607
Stojačić, Vlatka
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Stojačić, V. (2020). Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:838607

Stojačić, Vlatka. "Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:838607

Stojačić, Vlatka. "Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2020. https://urn.nsk.hr/urn:nbn:hr:152:838607

Stojačić, V. (2020). 'Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:838607

Stojačić V. Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2020 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:152:838607

V. Stojačić, "Antibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:152:838607

Metadata
TitleAntibakterijska aktivnost novosintetiziranih amidinobenzimidazolnih i kumarinskih spojeva
Title (english)Antibacterial activity of newly synthesized amidinobenzimidazole and coumarine compounds
AuthorVlatka Stojačić
MentorDomagoj Drenjančević (mentor)
Committee memberSuzana Mimica-Matanović (predsjednik povjerenstva)
Committee memberDubravka Lišnjić (član povjerenstva)
Committee memberDomagoj Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2020-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Medical Microbiology
Abstract
Cilj istraživanja: Svrha istraživanja bila je testirati osjetljivost multirezistentnih bakterija (Staphylococcus aureus ATCC (25923), meticilin – rezistentan S. aureus MRSA (11710), Escherichia coli ATCC (25922), Acinetobacter baumannii ATCC (19606), Acinetobacter baumannii (9768), Escherichia coli ESBL (26001)) na novosintetizirane amidinobenzimidazolne i kumarinske spojeve mikrodilucijskom metodom, interpretirati rezultate osjetljivosti i usporediti osjetljivost između bakterijskih vrsta. Materijali i metode: U istraživanju su korištene tri bakterijske vrste kliničkih sojeva (MRSA (11710), E. coli ESBL (26001), A. baumannii (9786)) i tri ATCC soja (A. baumannii ATCC (19606), S. aureus ATCC (25923), E. coli ATCC (25922)) te 29 amidinobenzimidazolnih i 29 kumarinskih spojeva. Nakon uzgoja, pripreme i inkubacije na Cation – adjusted Müller – Hinton bujonu u mikrotitarskim pločicama, očitani su rezultati mikrodilucijskog testa. Rezultati se očitavaju vizualno, usporedbom kontrolnih jažica spojeva i sojeva s testiranim jažicama, a očitava se minimalna inhibitorna koncentracija. Rezultati: Rezultati su pokazali da amidinobenzimidazolni spojevi imaju bolji inhibitorni učinak spram kumarinskih s naglaskom na gram – pozitivne bakterije (na S. aureus ATCC (25923) inhibitorno je djelovalo 8 (28 %) spojeva, 28 (97 %) kumarinskih spojeva nije pokazalo osjetljivost; na S. aureus MRSA (11710) inhibitornu aktivnost imalo je 11 (38 %) amidinobenzimidazola, a 25 (86 %) kumarinskih spojeva nije pokazalo osjetljivost. Kumarinski spojevi kod gram – negativnih bakterija nisu pokazali inhibitornu aktivnost, a djelovanje amidinobenzimidazolnih spojeva na E. coli ESBL (26001) obuhvaća raspon MIK 32 – 128 μg/mL, dok kod ostalih bakterija nisu očitane značajnije minimalne inhibitorne koncentracije. Zaključak: Rezultati ovog istraživanja ukazuju na nove mogućnosti de novo sintetiziranih spojeva kao potencijalnih antibakterijskih kemoterapeutika.
Abstract (english)
Objectives: The aim of this research is to examine the sensitivity of multi – resistant bacteria: Staphylococcus aureus ATCC 25923, methicillin – resistant S. aureus MRSA 11710, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606, Acinetobacter baumannii 9768, Escherichia coli ESBL 26001 to the newly synthesized amidinobenzimidazole and coumarin compounds by the microdilution method, as well as to interpret and compare sensitivity with other bacterial species. Material and methods: Three bacterial species from clinical specimens and three ATCC species with 29 amidinobenzimidazoles and 29 coumarins were used for this research. After cultivation, preparation and incubation in Cation-adjusted Müller – Hinton broth in the 96 – Well microtiter plates, microdilution test results were read visually, comparing control wells of compounds and strains with the tested wells. Minimal inhibitory concentrations was determined. Results: Results showed that amidinobenzimidazole compounds had a better inhibitory effect than coumarins with emphasis on gram – positive bacteria such as S. aureus ATCC 25923 (there were 8 (28 %) out 29 (100 %) amidinobenzimidazole compounds that showed sensitivity and 28 (97 %) coumarins did not show sensitivity) and methicilin – resistant S. aureus (MRSA) 11710 (11 (38 %) amidinobenzimidazoles showed sensitivity and 25 (86 %) coumarin did not). Coumarin compounds in gram – negative bacteria showed no inhibitory activity, but amidinobenzimidazole compounds have showed range of MICs 32 – 128 μg/mL, while ih other bacteria no significant minimum inhibitory concentrations were read. Conclusion: The results of this study indicate new possibilities of de novo synthesized compounds as potential antibacterial drugs.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:838607
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-12-17 13:30:16