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Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. urn:nbn:hr:152:182628
Krstanović, Ines
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

Cite this document

Krstanović, I. (2019). Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:182628

Krstanović, Ines. "Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:182628

Krstanović, Ines. "Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2019. https://urn.nsk.hr/urn:nbn:hr:152:182628

Krstanović, I. (2019). 'Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:182628

Krstanović I. Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2019 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:182628

I. Krstanović, "Utjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:152:182628

Metadata
TitleUtjecaj točkastih mutacija u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor na rizik za prijevremeni porod
Title (english)The influence of point mutations in exon 4 and 5 (H770H and V660L) of progesterone receptor gene on risk for premature delivery
AuthorInes Krstanović
MentorJasenka Wagner Kostadinović (mentor)
Committee memberMarija Heffer (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberJasenka Wagner Kostadinović (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2019-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Cilj istraživanja: Dokazati da su točkaste mutacije u egzonima 4 i 5 (V660L i H770H) gena za progesteronski receptor rizični čimbenici za prijevremeni porod. Ustroj studije: Retrospektivno istraživanje genetičkih varijacija u genu za progesteronski receptor koje mogu dovesti do prijevremenog poroda. Ispitanici i metode: Istraživanje je provedeno na rodiljama s Klinike za ginekologiju i opstetriciju KBC-a Osijek i njihovoj djeci. Skupljeno je 200 uzoraka. 100 uzoraka činilo je kontrolne skupine koje su se sastojale od terminskih trudnica (n = 50) i njihove djece (n = 50). 100 uzoraka činilo je ispitivane skupine u kojima su bile preterminske trudnice (n = 50) i njihova djeca (n = 50). Preterminske rodilje podijeljene su u sljedeće skupine: rani prijevremeni porodi (prije 28. gest. tjedna), umjereni prijevremeni porodi (28. - 32. gest. tjedan) te kasni prijevremeni porodi (32. - 37. gest. tjedan). Prvi korak u istraživanju bila je izolacija DNA pomoću komercijalnog kita NucleoSpin Blood iz uzoraka venske krvi majki i krvi iz pupčane vrpce djece. Drugi korak bila je genotipizacija DNA pomoću PCR-a u stvarnom vremenu prema protokolu Taqman SNP Genotyping Assay na uređaju Applied Biosystems. Upotrijebila se deskriptivna statistička obrada. Dobiveni rezultati statistički su obrađeni u programu MedCalc (inačica 16.2.0., MedCalc Software bvba, Ostend, Belgija). Rezultati: Ispitivane mutacije V660L i H770H nemaju statističku značajnost za prijevremeni porod. Isto vrijedi za dob i BMI. Zaključak: Istraživanje je pokazalo da mutacije V660L i H770H nisu rizični čimbenici za prijevremeni porod na ispitivanoj populaciji. Dob i BMI također nisu rizični čimbenici za prijevremeni porod.
Abstract (english)
Objectives: To prove that point mutations in exons 4 and 5 (V660L and H770H) of the progesterone receptor gene are risk factors for preterm delivery. Study design: A retrospective study of genetic variations in the progesterone receptor gene that may lead to preterm birth. Participants and methods: The study was conducted on postnatal mothers and their children at the Clinic for Gynaecology and Obstetrics of the Clinical Hospital Center Osijek. A total of 200 samples were collected. A 100 of them constituted control groups consisting of term mothers (n = 50) who gave birth spontaneously and their children (n = 50). The other 100 samples comprised the study groups consisting of preterm mothers (n = 50) and their children (n = 50). Preterm births were divided into the following groups: early preterm births (before 28th gestational week), moderate preterm births (28th - 32nd gestational weeks) and late preterm births (32nd - 37th gestational weeks). The first step in the study was DNA isolation using a commercial NucleoSpin Blood kit from maternal venous blood and from children blood samples from the umbilical cord. The second step was DNA genotyping using real-time PCR according to the TaqMan SNP Genotyping Assay protocol on an Applied Biosystems device. Descriptive statistical processing was performed. The obtained results were statistically processed in MedCalc (version 16.2.0., MedCalc Software bvba, Ostend, Belgium). Results: The tested V660L and H770H mutations have no statistical significance for premature birth. Also, age, BMI and p4 have no statistical significance. Conclusion: The study showed that the V660L and H770H mutations are not risk factors for preterm birth in the study population. Age and BMI are also not risk factors for premature birth.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:182628
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2021-05-10 08:53:14