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THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. urn:nbn:hr:152:330930
Kuna, Lucija
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Kuna, L. (2022). THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE (Doctoral thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:330930

Kuna, Lucija. "THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:330930

Kuna, Lucija. "THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE." Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:330930

Kuna, L. (2022). 'THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE', Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 10 May 2024, https://urn.nsk.hr/urn:nbn:hr:152:330930

Kuna L. THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE [Doctoral thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 May 10] Available at: https://urn.nsk.hr/urn:nbn:hr:152:330930

L. Kuna, "THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE", Doctoral thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:330930

Metadata
TitleTHE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE
Title (english)THE ACTIVITY OF GARLIC EXTRACTS TO THE EPITHELIAL DAMAGE CAUSED BY SODIUM TAUROCHOLATE IN A CELL CULTURE MODEL OF ULCER DISEASE
AuthorLucija Kuna
MentorRobert Smolić (mentor)
Committee memberMarija Heffer (predsjednik povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-02-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Ciljevi: Peptična ulkusna bolest (PUB) je kronična bolest koja pogađa do 10% svjetske populacije. Stanični model PUB-a može se uspostaviti u staničnom modelu ljudskih gastričnih epitelnih stanica (AGS) djelovanjem žučne soli; natrijevog taurokolata (NaT). Cilj istraživanja bio je istražiti učinke predtretmana ekstrakata češnjaka (EČ) i lanzoprazola (LPZ) u staničnom modelu ulkusne bolesti ispitivanjem oksidativnog stresa, distribucije F-aktina i određivanjem morfoloških promjena u strukturi stanične membrane. Ustroj studije: Stanice su nasađene u koncentraciji 4x105 stanica/mL te su potom inkubirane 24 sata. Stanice su potom tretirane LPZ i EČ 24, 48 i 72 sata i zatim izložene NaT tijekom 1 sata. Materijal i metode: AGS stanična linija korištena je kao model PUB. Uspostava NaT modela određena je MTT testom. Procjena je učinjena određivanjem mitotičkog potencijala stanica bojenjem proliferirajućeg staničnog jezgrenog antigena (PCNA); koncentracije glutationa (GSH) i prostaglandina E2 (PGE2) pomoću ELISA; učinka EČ na stanično preživljenje brojanjem stanica Neubauerovim hemocitometrom; ekspresije SOD, ABCG2, NFKB2 i TRX1 pomoću RT PCR-a; Vizualizacijom citoskeleta F-aktina polukvantificiranjem Rhodamine Phalloidin boje; morfoloških promjena u strukturi stanične membrane bojanjem kolesterola i fosfolipida specifičnim bojama. Rezultati: Rezultati naše studije pokazali su značajno smanjenje oštećenja stanica nakon inkubacije NAT-om, kada su AGS stanice prethodno tretirane LPZ-om (p <0,001) i povećanjem koncentracija EČ (p <0,001). Predtretman različitim koncentracijama EČ povećao je PGE2 i potisnuo iscrpljivanje GSH (p <0,001). Uočena je pozitivna korelacija SOD, NFKB2 (p <0,01) i TRX 1 (p <0,001) s LPZ i predtretmanom EČ, dok ekspresija ABCG2 nije promijenjena. (p <0,001). Predtretman stanica s EČ uzrokovao je promjene u sastavu stanične membrane i razine proteina citoskeleta izazvane izloženošću stanica NaT-u (p <0,001). Zaključak: Predtretman EČ imao je gastroprotektivni učinak u staničnom modelu PUB-i. Potrebni su daljnji eksperimenti kako bi se u potpunosti razjasnio mehanizam zaštitne uloge EČ u PUB-i.
Abstract (english)
Objectives: PUD is a chronic disease affecting up to 10% of the world's population. A cellular model of PUD can be established in AGS by NaT. The aim of the study was to explore effects of GE pretreatment and LPZ addition in the cell culture model of PUD by examining oxidative stress, F-actin distribution and determine morphological changes in cell membrane structure. Study design: Cells were plated at a density of 4x105 cells/mL in 6-well plates and were grown for 24 hours. Cells were pretreated with LPZ and GE for 24, 48 and 72 hours and subsequently exposed to NaT for 1 h. Material and methods: The AGS cell line was used as a model of PUD. The establishment of the NaT model was determined by the MTT test. Evaluation was done by determination of mitotic potential of the cells by by staining against (PCNA); GSH and PGE2 concentrations by ELISA; AGS proliferation by cell counting; expression of SOD, ABCG2, NFKB2 and TRX1 by RT PCR; F-actin cytoskeleton visualization by semi-quantification of Rhodamine Phalloidin stain; morphological changes in cell membrane structure by using CL and PH specific stains. Results: Our results showed significant reduction of cell damage after NaT incubation when the AGS cells were pretreated with LPZ (p<0.001) and increasing concentrations of GE (p<0.001). Pretreatment with different concentrations of GE increased PGE2 and suppressed depletion of GSH (p<0.001). Positive correlation of SOD, NFKB2 (p<0.01) and TRX 1 (p<0.001) with LPZ and GE pretreatment were seen, while ABCG2 expression was not changed. (p<0.001). Pretreatment of the cells with GE reverses changes in the cell membrane composition and cytoskeletal protein levels induced by NaT exposure (p<0.001). Conclusion: GE pretreatment had gastroprotective effect in the cell model of PUD. Further experiments are needed to fully elucidate the mechanism of the protective role of GE in PUD.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:330930
Promotion2022
Study programmeTitle: University Postgraduate Study
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
Extent112 str.; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-19 10:24:09