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master's thesis
Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. urn:nbn:hr:152:668208
Mikulec, Katarina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Mikulec, K. (2021). Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:668208

Mikulec, Katarina. "Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:668208

Mikulec, Katarina. "Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:668208

Mikulec, K. (2021). 'Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:668208

Mikulec K. Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:668208

K. Mikulec, "Utjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:668208

Metadata
TitleUtjecaj antihistaminika desloratadina i bilastina na razvoj mozga Sprague Dawley štakora
Title (english)Effect of desloratadine and bilastine antihistamines on brain development of Sprague Dawley rats
AuthorKatarina Mikulec
MentorMarija Heffer (mentor)
Committee memberBarbara Viljetić (predsjednik povjerenstva)
Committee memberInes Bilić-Ćurčić (član povjerenstva)
Committee memberMarija Heffer (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
Cilj istraživanja: Usporediti tri skupine potomaka netretiranih majki ili majki tretiranih antihistaminicima s obzirom na izražaj H1 receptora, markere neurona i glije te neuroplastin kao marker sinaptogeneze. Nacrt studije: U studiju je uključeno 36 životinja starosti 3 mjeseca. Životinje su podijeljene u 3 skupine: 1. potomci netretiranih Sprague Dawley štakorica (kontrola), 2. potomci štakorica tretiranih desloratadinom, 3. potomci štakorica tretiranih bilastinom. Materijali i metode: Histološkom bojom krezil violet prikazali smo jezgre stanica u mozgu te tako razaznali slojeve mozga. Imunohistokemijski, pomoću visokospecifičnih protutijela na H1 receptor, neuronski jezgrin protein, glijalni fibrilarni kiseli protein te neuroplastin 65, prikazali smo navedene epitope u tkivu mozga. Rezultati: Postoji statistički značajna razlika u izražaju H1R i Np65 u pojedinim područjima mozga štakora, zatim u udjelima slojeva u M1 području te u omjeru NeuN/GFAP u Au1 području moždane kore. Također, tretirane skupine pokazuju promjene u anatomiji hipokampusa. Zaključak: Antihistaminici bilastin i desloratadin djeluju na razvoj neurona i glije, izražaj H1R te sinaptogenezu u mozgu štakora.
Abstract (english)
Objectives: To compare three groups of cubs originating from untreated mothers or mothers treated with antihistamines with respect to H1 receptor expression, neuronal and glial markers, and neuroplastin as a marker of synaptogenesis. Study design: The study included 36 animals at the age of 3 months. Animals were divided into 3 groups: 1 offspring of untreated Sprague Dawley rats (control), 2 offspring of desloratadine-treated rats, 3 offspring of bilastine-treated rats. Material and methods: With the histological stain of cresyl violet we showed the cell nuclei in the brain and thus distinguished the layers of the brain. Immunohistochemically, using highly specific antibodies to the H1 receptor, neuronal nuclear protein, glial fibrillary acidic protein and neuroplastin 65, we presented these epitopes in brain tissue. Results: The results showed statistically significant differences in the expression of H1R and Np65 in individual areas of the rat brain, also in the proportions of layers in the M1 region and in the NeuN/GFAP ratio in the Au1 region of the cerebral cortex. Also, the treated groups show changes in the anatomy of the hippocampus. Conclusion: The antihistamines bilastine and desloratadine act on the development of neurons and glia, H1R expression and synaptogenesis in the rat brain.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:668208
Study programmeTitle: University Graduate Studies
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-12-19 12:50:26