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master's thesis
Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. urn:nbn:hr:152:621397
Živatović, Karlo
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Živatović, K. (2021). Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:621397

Živatović, Karlo. "Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:621397

Živatović, Karlo. "Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:621397

Živatović, K. (2021). 'Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 15 June 2024, https://urn.nsk.hr/urn:nbn:hr:152:621397

Živatović K. Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 June 15] Available at: https://urn.nsk.hr/urn:nbn:hr:152:621397

K. Živatović, "Procjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:621397

Metadata
TitleProcjena antiproliferativne aktivnosti novosintetiziranih benzotiazola kao aktivatora apoptoze
Title (english)Evaluation of antiproliferative activity of newly synthesized benzothiazoles as apoptosis activators
AuthorKarlo Živatović
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberKatarina Mišković Špoljarić (predsjednik povjerenstva)
Committee memberMarijana Leventić (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Human Genetics, Genomics and Proteomics
Abstract
Ciljevi istraživanja: Ispitati proapoptotski potencijal derivata benzotiazola na stanice limfoma. Nacrt studije: Ovaj diplomski rad organiziran je kao pokusno (eksperimentalno) istraživanje. Materijal i metode: U istraživanju je korišteno šest različitih derivata benzotiazola: D1, D2, D3, D4, D5 i D6. Antiproliferativno djelovanje derivata benzotiazola ispitivano je na humanoj staničnoj liniji HuT-78 koja se sastoji od tumorskih stanica T – staničnog limfoma. Stanice su se uzgajale in vitro i održavale u bocama za kultivaciju te bojale eritrozinom B kako bi se odredio broj živih stanica. Stanice su tretirane 5 μmol/L derivatima benzotiazola tijekom 24 sata. Nakon tretmana stanice su bojane aneksinom V konjugiranim s Alexa Fluor 488 bojom i propidij jodidom za određivanje stanica u apoptozi, te JC-1 bojom pomoću koje je određen postotak stanica s narušenim mitohondrijskim membranskim potencijalom. Dobiveni rezultati analizirani su pomoću statističkog programa XLSTAT 2021.2.2 za Windows operativne sustave. Rezultati: Svi ispitivani derivati uzrokuju povećanje udjela apoptotskih stanica u tretiranim HuT-78 stanicama. Derivati D1 i D3 uzrokuju povećanje postotka stanica s narušenim mitohondrijskim membranskim potencijalom. Statistička obrada podataka pokazuje da nastale promjene nisu statistički značajne. Zaključak: Svi ispitivani derivati benzotiazola pokazali su minimalni proapoptotski učinak na testirane stanice.
Abstract (english)
Objectives: The goal of this study is to test the proapoptotic potential of newly synthesized benzothiazole derivatives on T-cell lymphoma HuT-78 cell line. Study design: This master’s thesis is organized as an experimental research. Material and Methods: There were six different benzothiazole derivatives used in the research: D1, D2, D3, D4, D5 i D6. Anitproliferative activity of benzothiazole derivatives has been tested on T-cell lymphoma HuT-78 cell line. Cells have been growned and maintained in vitro in specialized bottles for cell cultivation. After that, they have been stained with erythrosine B to determine the number of living cells. Cells have been treated with 5 μmol/L benzothiazole derivatives during 24 hours. After the treatment cells have been stained with annexin V that's been conjugated with Alexa Fluor 488 stain and with propidium iodide to determine the percentage of cells in apoptosis. Percentage of cells with disturbed mitochondrial membrane potential has been determined with JC-1 stain. Final results have been analysed with XLSTAT 2021.2.2 analytics software. Results: All of the tested benzothiazole derivatives induced increase number of apoptotic cells compared to the untreated control. D1 and D3 derivatives showed greater percentage of cells with ruined mitochondrial membrane potential. Statistical evaluation of the results has shown that none of the changes are statistically significant. Conclusion: All of the benzothiazole derivatives that have been researched showed minimal proapoptotic effect on the tested cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:621397
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2022-12-21 10:48:10