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undergraduate thesis
Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. urn:nbn:hr:152:878902
Tomić, Nikolina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Tomić, N. (2021). Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:878902

Tomić, Nikolina. "Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:878902

Tomić, Nikolina. "Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2021. https://urn.nsk.hr/urn:nbn:hr:152:878902

Tomić, N. (2021). 'Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:878902

Tomić N. Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2021 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:152:878902

N. Tomić, "Udio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:152:878902

Metadata
TitleUdio apoptotskih stanica u uzorcima 3D sferoida glioblastoma različite veličine
Title (english)Apoptotic cells proportion in glioblastoma 3D spheroid samples of different sizes
AuthorNikolina Tomić
MentorTeuta Opačak-Bernardi (mentor)
Committee memberMarijana Leventić (predsjednik povjerenstva)
Committee memberBarbara Viljetić (član povjerenstva)
Committee memberTeuta Opačak-Bernardi (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2021-09-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj istraživanja: Odrediti udio apoptotskih stanica u 3D sferoidima glioblastoma formiranim metodom magnetske levitacije nakon 5, 10 i 15 dana rasta. Nacrt studije: Istraživanje je osmišljeno kao pokusno. Materijali i metode: Udio apoptoze nakon 5, 10 i 15 dana rasta ispitan je na stanicama glioblastoma (stanična linija D54). Sferoidi su formirani metodom magnetske levitacije. Udio apoptotskih stanica analiziran je na protočnom citometru uz prethodno bojanje stanica Annexin V FITC-om i propidij jodidom. Rezultati: Tijekom vremena smanjen je udio proliferirajućih stanica. Udio nekrotičnih stanica s vremenom se povećao. Ne postoji značajna razlika u udjelu apoptotskih stanica između 5. i 15. dana rasta. Zaključak: Duži period rasta ne rezultira povećanjem udjela apoptotskih stanica, nego se povećava udio stanica u nekrozi.
Abstract (english)
Objectives: The aim of this study is to analyse apoptotic cell proportion in glioblastoma 3D spheroids that were formed using magnetic levitation method after 5,10 and 15 days of growth. Study Design: The study was designed as experimental. Materials and Methods: Apoptotic cell proportion after 5, 10 and 15 days of growth is tested on glioblastoma cells (cell line D54). Spheroids are formed using magnetic levitation method. Apoptotic cell proportion was analysed on a flow cytometer with prior staining of the cells with Annexin V FITC and propidium iodide. Results: Proportion of proliferating cells has been reduced, as oppose to proportion of necrotic cells that has been increased over time. There is no significant difference in apoptotic cell proportion between 5th and 15th day of growth. Conclusion: Longer period of growth does not result in increased proportion of apoptotic cells, but the proportion of necrotic cells grows in corelation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:878902
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2023-01-02 09:00:29