prikaz prve stranice dokumenta Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode
  Download
PDF 1.04 MB
master's thesis
Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. urn:nbn:hr:152:127163
Grgić, Zvonimir
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

Cite this document

Grgić, Z. (2022). Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:127163

Grgić, Zvonimir. "Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:127163

Grgić, Zvonimir. "Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2022. https://urn.nsk.hr/urn:nbn:hr:152:127163

Grgić, Z. (2022). 'Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:127163

Grgić Z. Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:127163

Z. Grgić, "Analiza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:152:127163

Metadata
TitleAnaliza raznolikosti beta lanca T staničnih receptora u TCRV alfa 7.2 + MR1+ MAIT stanicama oboljelih od vulgarne psorijaze primjenom RNASeq metode
Title (english)Analysis of T cell receptor beta chain diversity in TCRValpha7.2+ MR1+ MAIT cells of psoriasis vulgaris patients using RNASeq methodology
AuthorZvonimir Grgić
MentorStana Tokić (mentor)
Committee memberMario Štefanić (predsjednik povjerenstva)
Committee memberJasenka Wagner Kostadinović (član povjerenstva)
Committee memberStana Tokić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2022-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Neuroscience
Abstract
Uvod: Psorijaza je kronična, autoimuna bolest kože s jakom genskom podlogom. MAIT stanice su nekonvencionalni limfociti T uključeni u imunosni odgovor u psorijazi. One izražavaju semi-invarijantni TCRVα7.2β receptor, koji prepoznaje nepeptidne antigene u kompleksu s MR1 receptorom. Aktivirane MAIT stanice luče citokine IL-17, IFN-y i TNF-α pokazujući citotoksična svojstva. Ciljevi istraživanja: Istražiti i usporediti repertoar, identificirati oligoklonalne varijante β-TCR klonotipova perifernih TCRVα7.2+MR1+ MAIT stanica te ustanoviti njihovu povezanost s težinom i obilježjima bolesti. Materijali i metode: Istraživanjem su prikupljeni uzorci periferne krvi 10 oboljelih (PV) i 10 zdravih (CTRL) ispitanika. Sortirane linije perifernih TCRVα7.2+MR1+ MAIT stanica su kvantificirane („Qubit™ RNA HS Assay Kit“), razrijeđene te je sekvenciranje (Illumina® MiniSeq™) provedeno nakon pripreme NGS knjižnice. Podatci su obrađeni i analizirani primjenom MIXCR, Immunarch i VDJTools alata. Rezultati: U usporedbi sa zdravim ispitanicima, oboljeli od psorijaze imaju značajno veću učestalost upotrebe gena TRBV29-1, TRBV11-2, TRBV7-6 i TRBV6-9 u repertoaru TCRβ lanaca MAIT stanica. Zabilježena je povezanost veličine PASI indeksa i klonalne zastupljenosti TRBV21-1, TRBJ1-1, TRBV11-1, TRBV28 i TRBV6-5 gena. Zaključak: Oboljeli od psorijaze pokazuju veću učestalost upotrebe gena TRBV29-1, TRBV11-2, TRBV7-6 i TRBV6-9 u klonalnom repertoaru TCRβ lanaca MAIT stanica. Klonalna zastupljenost TRBV21-1, TRBJ1-1, TRBV11-1, TRBV28 i TRBV6-5 gena u psorijazi povezana je s težinom oboljenja.
Abstract (english)
Introduction: Psoriasis is chronic, autoimmune skin disease with strong genetic background. MAIT cells are unconventional T lymphocytes involved in immune response in psoriasis. They express semi-invariant TCRVα7.2β receptor which recognizes non-peptid antigens presented by MR1. Upon activation, they secrete cytokines IL-17, IFN-y and TNF-α suggesting cytotoxic effector function. Objectives: To explore and compare β -TCR clonotype of peripheral TCRVα7.2+MR1+ MAIT cell repertoire, identify oligoclonal variants and determine their association with clinical features and severity of psoriasis. Materials and methods: Peripheral blood was collected from 10 PV patients and 10 healthy controls. TCRVα7.2+MR1+ MAIT sorted cells were quantified with Qubit™ RNA HS Assay Kit, diluted and sequenced on Illumina® MiniSeq™ after preparing NGS library. MiXCR, Immunarch and VDJTools were used for dana analysis. Results: Compared to the control subjects, PV patients have had significantly increased TRBV29-1, TRBV11-2, TRBV7-6 and TRBV6-9 gene usage in MAIT cell TCRβ repertoire. Significant correlation has been observed between PASI and TRBV21-1, TRBJ1-1, TRBV11-1, TRBV28 and TRBV6-5 genes clonal distribution. Conclusion: PV patients have shown increased TRBV29-1, TRBV11-2, TRBV7-6 and TRBV6-9 gene usage in MAIT cell TCRβ repertoire. Clonal distribution of TRBV21-1, TRBJ1-1, TRBV11-1, TRBV28 and TRBV6-5 genes in psoriasis was associated with disease severity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:127163
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-07-21 07:52:39